Anti-P114-RHO-GEF/ARHGEF18 antibody (C-Term) (STJ70465)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-P114-RHO-GEF/ARHGEF18 (C-Term) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications. |
| Applications | Pep-ELISA/WB/IF |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 140+90kDa band observed in lysates of cell line Daudi and Caco-2 (calculated MW of 114kDa according to NP_056133.2 and 131kDa accoeding to NP_001124427.1). Recommended concentr |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | ARHGEF18 |
| Gene ID | 23370 |
| Uniprot ID | ARHGI_HUMAN |
| Accession Number | NP_056133.2; NP_001124427.1 |
| Immunogen Region | C-Term |
| Immunogen Sequence | SAKEDASKEDVIFF |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_056133.2; NP_001124427.1). |
Additional Info
| Function | Acts as a guanine nucleotide exchange factor (GEF) for RhoA GTPases. Its activation induces formation of actin stress fibers. Also acts as a GEF for RAC1, inducing production of reactive oxygen species (ROS). Does not act as a GEF for CDC42. The G protein beta-gamma (Gbetagamma) subunits of heterotrimeric G proteins act as activators, explaining the integrated effects of LPA and other G-protein coupled receptor agonists on actin stress fiber formation, cell shape change and ROS production. Required for EPB41L4B-mediated regulation of the circumferential actomyosin belt in epithelial cells. |
| Protein Name | Rho Guanine Nucleotide Exchange Factor 18114 Kda Rho-Specific Guanine Nucleotide Exchange FactorP114-Rho-GefP114rhogefSeptin-Associated RhogefSa-Rhogef |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-2173791Reactome: R-HSA-416482Reactome: R-HSA-8980692Reactome: R-HSA-9013149 |
| Cellular Localisation | CytoplasmCytoskeletonCell MembraneApical Cell MembraneIn Unactivated EosinophilsDistributed Around The Cell Periphery In The Perimembranous RegionIn Activated EosinophilsRelocates To The Tip Of The NucleopodA Membrane Structure Formed During Activation When The Nucleus Moves To One End Of The CellAnd Is Also Concentrated In Membrane Protrusions At The Opposite End Of The CellLocalizes To The Apical Cell Membrane In Epithelial Cells |
| Alternative Antibody Names | Anti-Rho Guanine Nucleotide Exchange Factor 18 antibodyAnti-114 Kda Rho-Specific Guanine Nucleotide Exchange Factor antibodyAnti-P114-Rho-Gef antibodyAnti-P114rhogef antibodyAnti-Septin-Associated Rhogef antibodyAnti-Sa-Rhogef antibodyAnti-ARHGEF18 antibodyAnti-KIAA0521 antibody |
Information sourced from Uniprot.org