• Western blot analysis of various lysates using OXCT1 Rabbit polyclonal antibody (STJ11104584) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-OXCT1 antibody (261-520) (STJ11104584)

SKU:
STJ11104584

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Succinyl-Coa-3-Ketoacid Coenzyme A Transferase 1-Mitochondrial (261-520) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OXCT1
Gene ID: 5019
Uniprot ID: SCOT1_HUMAN
Immunogen Region: 261-520
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 261-520 of human OXCT1 (NP_000427.1).
Immunogen Sequence: IPQIYVHRLIKGEKYEKRIE RLSIRKEGDGEAKSAKPGDD VRERIIKRAALEFEDGMYAN LGIGIPLLASNFISPNITVH LQSENGVLGLGPYPRQHEAD ADLINAGKETVTILPGASFF SSDESFAMIRGGHVDLTMLG AMQVSKYGDLANWMIPGKMV KGMGGAMDLVSSAKTKVVVT MEHSAKGNAHKIMEKCTLPL TGKQCVNRIITEKAVFDVDK KKGLTLIELWEGLTVDDVQ
Tissue Specificity Abundant in heart, followed in order by brain, kidney, skeletal muscle, and lung, whereas in liver it is undetectable. Expressed (at protein level) in all tissues (except in liver), most abundant in myocardium, then brain, kidney, adrenal glands, skeletal muscle and lung.also detectable in leukocytes and fibroblasts.
Function Key enzyme for ketone body catabolism. Catalyzes the first, rate-limiting step of ketone body utilization in extrahepatic tissues, by transferring coenzyme A (CoA) from a donor thiolester species (succinyl-CoA) to an acceptor carboxylate (acetoacetate), and produces acetoacetyl-CoA. Acetoacetyl-CoA is further metabolized by acetoacetyl-CoA thiolase into two acetyl-CoA molecules which enter the citric acid cycle for energy production. Forms a dimeric enzyme where both of the subunits are able to form enzyme-CoA thiolester intermediates, but only one subunit is competent to transfer the CoA moiety to the acceptor carboxylate (3-oxo acid) and produce a new acyl-CoA. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
Protein Name Succinyl-Coa -3-Ketoacid Coenzyme A Transferase 1 - Mitochondrial
Scot
3-Oxoacid Coa-Transferase 1
Somatic-Type Succinyl-Coa -3-Oxoacid Coa-Transferase
Scot-S
Succinyl-Coa -3-Oxoacid Coa Transferase
Database Links Reactome: R-HSA-77108
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Succinyl-Coa -3-Ketoacid Coenzyme A Transferase 1 - Mitochondrial antibody
Anti-Scot antibody
Anti-3-Oxoacid Coa-Transferase 1 antibody
Anti-Somatic-Type Succinyl-Coa -3-Oxoacid Coa-Transferase antibody
Anti-Scot-S antibody
Anti-Succinyl-Coa -3-Oxoacid Coa Transferase antibody
Anti-OXCT1 antibody
Anti-OXCT antibody
Anti-SCOT antibody

Information sourced from Uniprot.org

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