| Host: | Goat |
| Applications: | Pep-ELISA/IF/FC |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-OTUD4 (C-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary testing showed a band at approx. 110kDa in Human Skeletal Muscle lysate after 2ug/ml antibody staining. (calculated MW of 117kDa according to NP_955356.1). This band was suc |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | OTUD4 |
| Gene ID: | 54726 |
| Uniprot ID: | OTUD4_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_955356.1; NP_001096123.1 |
| Specificity: | This antibody is expected to recognize reported isoforms 1 (NP_955356.1) and 3 (NP_001096123.1). |
| Immunogen Sequence: | SRSRDEGYQYHRNVR |
| Function | Deubiquitinase which hydrolyzes the isopeptide bond between the ubiquitin C-terminus and the lysine epsilon-amino group of the target protein. May negatively regulate inflammatory and pathogen recognition signaling in innate immune response. Upon phosphorylation at Ser-202 and Ser-204 residues, via IL-1 receptor and Toll-like receptor signaling pathway, specifically deubiquitinates 'Lys-63'-polyubiquitinated MYD88 adapter protein triggering down-regulation of NF-kappa-B-dependent transcription of inflammatory mediators. Independently of the catalytic activity, acts as a scaffold for alternative deubiquitinases to assemble specific deubiquitinase-substrate complexes. Associates with USP7 and USP9X deubiquitinases to stabilize alkylation repair enzyme ALKBH3, thereby promoting the repair of alkylated DNA lesions. |
| Protein Name | Otu Domain-Containing Protein 4Hiv-1-Induced Protein Hin-1 |
| Cellular Localisation | CytoplasmNucleusPrimarily Cytoplasmic |
| Alternative Antibody Names | Anti-Otu Domain-Containing Protein 4 antibodyAnti-Hiv-1-Induced Protein Hin-1 antibodyAnti-OTUD4 antibodyAnti-HIN-1 antibodyAnti-KIAA1046 antibody |
Information sourced from Uniprot.org