• Western blot analysis of lysates from wild type (WT) and 293T cells transfected with OTC rabbit polyclonal antibody, using OTC rabbit polyclonal antibody (STJ111876) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates, using OTC rabbit polyclonal antibody (STJ111876) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-OTC antibody (215-354) (STJ111876)

SKU:
STJ111876

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OTC (215-354) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:4000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OTC
Gene ID: 5009
Uniprot ID: OTC_HUMAN
Immunogen Region: 215-354
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 215-354 of human OTC (NP_000522.3).
Immunogen Sequence: LQAATPKGYEPDASVTKLAE QYAKENGTKLLLTNDPLEAA HGGNVLITDTWISMGQEEEK KKRLQAFQGYQVTMKTAKVA ASDWTFLHCLPRKPEEVDDE VFYSPRSLVFPEAENRKWTI MAVMVSLLTDYSPQLQKPKF
Tissue Specificity Mainly expressed in liver and intestinal mucosa.
Post Translational Modifications Acetylation at Lys-88 negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals.
Function Catalyzes the second step of the urea cycle, the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline. The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion.
Protein Name Ornithine Transcarbamylase - Mitochondrial
Otcase
Ornithine Carbamoyltransferase - Mitochondrial
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-70635
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Ornithine Transcarbamylase - Mitochondrial antibody
Anti-Otcase antibody
Anti-Ornithine Carbamoyltransferase - Mitochondrial antibody
Anti-OTC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance