• Western blot analysis of extracts of various cell lines, using OSTF1 antibody (STJ114062) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-OSTF1 antibody (15-214) (STJ114062)

SKU:
STJ114062

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OSTF1 (15-214) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OSTF1
Gene ID: 26578
Uniprot ID: OSTF1_HUMAN
Immunogen Region: 15-214
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 15-214 of human OSTF1 (NP_036515.4).
Immunogen Sequence: KVFRALYTFEPRTPDELYFE EGDIIYITDMSDTNWWKGTS KGRTGLIPSNYVAEQAESID NPLHEAAKRGNLSWLRECLD NRVGVNGLDKAGSTALYWAC HGGHKDIVEMLFTQPNIELN QQNKLGDTALHAAAWKGYAD IVQLLLAKGARTDLRNIEKK LAFDMATNAACASLLKKKQG TDAVRTLSNAEDYLDDEDSD
Tissue Specificity Ubiquitously expressed. Present in osteoclasts (at protein level).
Function Induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity.
Protein Name Osteoclast-Stimulating Factor 1
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Osteoclast-Stimulating Factor 1 antibody
Anti-OSTF1 antibody

Information sourced from Uniprot.org

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