• Anti-Osteopontin/OPN/SPP1 antibody (17-294) (STJ11105182)
  • Immunofluorescence analysis of paraffin-embedded Mouse kidney tissue using Osteopontin/OPN/SPP1 Rabbit polyclonal antibody (STJ11105182) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of lysates from RAW264. 7 cells, using Osteopontin/OPN/SPP1 Rabbit polyclonal antibody (STJ11105182) at 1:700 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Osteopontin/OPN/SPP1 antibody (17-294) (STJ11105182)

SKU:
STJ11105182

$151.88 - $705.38
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Osteopontin/OPN/SPP1 (17-294) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Spp1
Gene ID: 20750
Uniprot ID: OSTP_MOUSE
Immunogen Region: 17-294
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 17-294 of mouse osteopontin/OPN/SPP1 (NP_001191162.1).
Immunogen Sequence: LPVKVTDSGSSEEKLYSLHP DPIATWLVPDPSQKQNLLAP QNAVSSEEKDDFKQETLPSN SNESHDHMDDDDDDDDDDGD HAESEDSVDSDESDESHHSD ESDETVTASTQADTFTPIVP TVDVPNGRGDSLAYGLRSKS RSFQVSDEQYPDATDEDLTS HMKSGESKESLDVIPVAQLL SMPSDQDNNGKGSHESSQLD EPSLETHRLEHSKESQESAD QSDVIDSQASSKASLEHQS
Post Translational Modifications Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif. The phosphorylated form inhibits hydroxyapatite crystallization. Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization. O-glycosylated. Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties.
Function Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Protein Name Osteopontin
2ar
Bone Sialoprotein 1
Calcium Oxalate Crystal Growth Inhibitor Protein
Early T-Lymphocyte Activation 1 Protein
Minopontin
Secreted Phosphoprotein 1
Spp-1
Database Links Reactome: R-MMU-1474228
Reactome: -MMU-186797
Reactome: -MMU-216083
Reactome: -MMU-381426
Reactome: -MMU-8957275
Cellular Localisation Secreted
Alternative Antibody Names Anti-Osteopontin antibody
Anti-2ar antibody
Anti-Bone Sialoprotein 1 antibody
Anti-Calcium Oxalate Crystal Growth Inhibitor Protein antibody
Anti-Early T-Lymphocyte Activation 1 Protein antibody
Anti-Minopontin antibody
Anti-Secreted Phosphoprotein 1 antibody
Anti-Spp-1 antibody
Anti-Spp1 antibody
Anti-Eta-1 antibody
Anti-Op antibody
Anti-Spp-1 antibody

Information sourced from Uniprot.org

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