| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-OSBP (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 85kDa band observed in Human Placenta lysates (calculated MW of 89.4kDa according to NP_002547). Recommended concentration: 0.1-0.3µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | OSBP |
| Gene ID: | 5007 |
| Uniprot ID: | OSBP1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_002547.1 |
| Immunogen Sequence: | KHQLEETKKEKRTRIP |
| Function | Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum: specifically exchanges sterol with phosphatidylinositol 4-phosphate (PI4P), delivering sterol to the Golgi in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability. |
| Protein Name | Oxysterol-Binding Protein 1 |
| Database Links | Reactome: R-HSA-1660661Reactome: R-HSA-192105 |
| Cellular Localisation | CytoplasmCytosolPerinuclear RegionGolgi Apparatus MembranePeripheral Membrane ProteinEndoplasmic Reticulum MembraneGolgi ApparatusTrans-Golgi NetworkPredominantly Cytosolic |
| Alternative Antibody Names | Anti-Oxysterol-Binding Protein 1 antibodyAnti-OSBP antibodyAnti-OSBP1 antibody |
Information sourced from Uniprot.org