• Immunofluorescence analysis of U2OS cells using ODC1 Rabbit polyclonal antibody (STJ24858) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using ODC1 Rabbit polyclonal antibody (STJ24858) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using ODC1 Rabbit polyclonal antibody (STJ24858) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ODC1 Rabbit polyclonal antibody (STJ24858) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ODC1 antibody (1-280) (STJ24858)

SKU:
STJ24858

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ODC1
Gene ID: 4953
Uniprot ID: DCOR_HUMAN
Immunogen Region: 1-280
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ODC1 (NP_002530.1).
Immunogen Sequence: MNNFGNEEFDCHFLDEGFTA KDILDQKINEVSSSDDKDAF YVADLGDILKKHLRWLKALP RVTPFYAVKCNDSKAIVKTL AATGTGFDCASKTEIQLVQS LGVPPERIIYANPCKQVSQI KYAANNGVQMMTFDSEVELM KVARAHPKAKLVLRIATDDS KAVCRLSVKFGATLRTSRLL LERAKELNIDVVGVSFHVGS GCTDPETFVQAISDARCVFD MGAEVGFSMYLLDIGGGFP
Post Translational Modifications S-Nitrosylation inhibits the enzyme. S-Nitrosylated in vitro on 4 cysteine residues.
Function Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis.
Protein Name Ornithine Decarboxylase
Odc
Database Links Reactome: R-HSA-350562
Reactome: R-HSA-351202
Alternative Antibody Names Anti-Ornithine Decarboxylase antibody
Anti-Odc antibody
Anti-ODC1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance