| Host: |
Goat |
| Applications: |
Pep-ELISA/WB |
| Reactivity: |
Human/Mouse/Rat/Dog |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-OCT4/POU5F1 (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 30kDa band observed in Human Placenta, and approx 28kDa in Mouse Heart lysates (calculated MW of 30.1kDa according to Human NP_976034.3 and 25.0kDa according to Mouse NP_00123938 |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
POU5F1 |
| Gene ID: |
5460 |
| Uniprot ID: |
PO5F1_HUMAN |
| Immunogen Region: |
C-Term |
| Accession Number: |
NP_002692.2; NP_976034.3 |
| Specificity: |
This antibody is expected to recognise both reported isoforms (NP_002692.2 and NP_976034.3) |
| Immunogen Sequence: |
C-VTTLGSPMHSN |
| Post Translational Modifications | Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression. Ubiquitinated.undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation. ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription. |
| Function | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. |
| Protein Name | Pou Domain - Class 5 - Transcription Factor 1Octamer-Binding Protein 3Oct-3Octamer-Binding Protein 4Oct-4Octamer-Binding Transcription Factor 3Otf-3 |
| Database Links | Reactome: R-HSA-2892245Reactome: R-HSA-2892247Reactome: R-HSA-452723Reactome: R-HSA-9754189Reactome: R-HSA-9823739Reactome: R-HSA-9827857Reactome: R-HSA-9834899 |
| Cellular Localisation | CytoplasmNucleusExpressed In A Diffuse And Slightly Punctuate PatternColocalizes With Mapk8 And Mapk9 In The Nucleus |
| Alternative Antibody Names | Anti-Pou Domain - Class 5 - Transcription Factor 1 antibodyAnti-Octamer-Binding Protein 3 antibodyAnti-Oct-3 antibodyAnti-Octamer-Binding Protein 4 antibodyAnti-Oct-4 antibodyAnti-Octamer-Binding Transcription Factor 3 antibodyAnti-Otf-3 antibodyAnti-POU5F1 antibodyAnti-OCT3 antibodyAnti-OCT4 antibodyAnti-OTF3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
