• Immunofluorescence analysis of Human-breast-cancer tissue. 1, Oct1 monoclonal antibody (7G1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Western blot analysis of 1) Hela, 2) Jurkat, 3) HepG2, diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1, Oct1 monoclonal antibody (7G1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Oct1 antibody [7G1] (STJ96991)

SKU:
STJ96991

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-POU domain, class 2, transcription factor 1 and POU domain, class 2, transcription factor 2 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: 7G1
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 1:500-2000
IF 1:200
IHC 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POU2F1
POU2F2
Gene ID: 5451
5452
Uniprot ID: PO2F1_HUMAN
PO2F2_HUMAN
Specificity: The antibody detects endogenous Oct1 protein.
Immunogen: Synthetic Peptide of 42644
Post Translational Modifications Phosphorylated by PRKDC.
Function Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR. (Microbial infection) In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
Protein Name Pou Domain - Class 2 - Transcription Factor 1
Nf-A1
Octamer-Binding Protein 1
Oct-1
Octamer-Binding Transcription Factor 1
Otf-1
Database Links Reactome: R-HSA-6785807
Reactome: R-HSA-6807505
Reactome: R-HSA-749476
Reactome: R-HSA-76071
Reactome: R-HSA-9018519
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pou Domain - Class 2 - Transcription Factor 1 antibody
Anti-Nf-A1 antibody
Anti-Octamer-Binding Protein 1 antibody
Anti-Oct-1 antibody
Anti-Octamer-Binding Transcription Factor 1 antibody
Anti-Otf-1 antibody
Anti-POU2F1 antibody
Anti-OCT1 antibody
Anti-OTF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance