• Immunofluorescence analysis of paraffin-embedded mouse heart using OBSCN Rabbit polyclonal antibody (STJ11100081) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded rat heart using OBSCN Rabbit polyclonal antibody (STJ11100081) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using OBSCN Rabbit polyclonal antibody (STJ11100081) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-OBSCN antibody (1621-1710) (STJ11100081)

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STJ11100081

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-OBSCN (1621-1710) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: OBSCN
Gene ID: 84033
Uniprot ID: OBSCN_HUMAN
Immunogen Region: 1621-1710
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1621-1710 of human OBSCN (NP_443075.3).
Immunogen Sequence: EPKVVFAKEQPAHREVQAEA GASATLSCEVAQAQTEVTWY KDGKKLSSSSKVRVEAVGCT RRLVVQQAGQAEAGEYSCEA GGQQLSFRLQ
Post Translational Modifications Autophosphorylated by protein kinase domains 1 and 2.
Function Structural component of striated muscles which plays a role in myofibrillogenesis. Probably involved in the assembly of myosin into sarcomeric A bands in striated muscle. Has serine/threonine protein kinase activity and phosphorylates N-cadherin CDH2 and sodium/potassium-transporting ATPase subunit ATP1B1. Binds (via the PH domain) strongly to phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), and to a lesser extent to phosphatidylinositol 3-phosphate (PtdIns(3)P), phosphatidylinositol 4-phosphate (PtdIns(4)P), phosphatidylinositol 5-phosphate (PtdIns(5)P) and phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3).
Protein Name Obscurin
Obscurin-Rhogef
Obscurin-Myosin Light Chain Kinase
Obscurin-Mlck
Database Links Reactome: R-HSA-193648
Reactome: R-HSA-416482
Reactome: R-HSA-8980692
Reactome: R-HSA-9013406
Cellular Localisation Isoform 3: Cytoplasm
Myofibril
Sarcomere
M Line
Cytoplasm
Z Line
In Differentiating Skeletal Muscle Cells
Isoform 3 Primarily Localizes To The Sarcomeric M-Line And Less Frequently To The Z-Disk
Isoform 3 Colocalizes With Ank1 Isoform Mu17/Ank1
5 At The M-Line In Differentiated Skeletal Muscle Cells
Cell Membrane
Sarcolemma
Nucleus
Colocalizes With Cdh2 And Atp1b1 To The Sarcolemma And To Intercalating Disks In Cardiac Muscles
Colocalizes With Atp1b1 To M Line And Z Line In Cardiac Muscles
Alternative Antibody Names Anti-Obscurin antibody
Anti-Obscurin-Rhogef antibody
Anti-Obscurin-Myosin Light Chain Kinase antibody
Anti-Obscurin-Mlck antibody
Anti-OBSCN antibody
Anti-KIAA1556 antibody
Anti-KIAA1639 antibody

Information sourced from Uniprot.org

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