• Immunohistochemistry analysis of paraffin-embedded mouse lung using NYX antibody (STJ110140) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human prostate using NYX antibody (STJ110140) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of 293T cells, using NYX antibody (STJ110140) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-NYX antibody (282-481) (STJ110140)

SKU:
STJ110140

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NYX
Gene ID: 60506
Uniprot ID: NYX_HUMAN
Immunogen Region: 282-481
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 282-481 of human NYX (NP_072089.1).
Immunogen Sequence: LLYLDRNSIAFVEEGAFQNL SGLLALHLNGNRLTVLAWVA FQPGFFLGRLFLFRNPWCCD CRLEWLRDWMEGSGRVTDVP CASPGSVAGLDLSQVTFGRS SDGLCVDPEELNLTTSSPGP SPEPAATTVSRFSSLLSKLL APRVPVEEAANTTGGLANAS LSDSLSSRGVGGAGRQPWFL LASCLLPSVAQHVVFGLQMD
Tissue Specificity Expressed in kidney and retina. Also at low levels in brain, testis and muscle. Within the retina, expressed in the inner segment of photoreceptors, outer and inner nuclear layers and the ganglion cell layer.
Protein Name Nyctalopin
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Nyctalopin antibody
Anti-NYX antibody
Anti-CLRP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance