• Immunofluorescence analysis of L929 cells using NXN Rabbit polyclonal antibody (STJ11102879) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using NXN Rabbit polyclonal antibody (STJ11102879) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using NXN Rabbit polyclonal antibody (STJ11102879) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Rat testis, using NXN Rabbit polyclonal antibody (STJ11102879) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NXN antibody (100-280) (STJ11102879)

SKU:
STJ11102879

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NXN (100-280) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NXN
Gene ID: 64359
Uniprot ID: NXN_HUMAN
Immunogen Region: 100-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-280 of human NXN (NP_001192248.1).
Immunogen Sequence: CRSLTRVLVESYRKIKEAGQ NFEIIFVSADRSEESFKQYF SEMPWLAVPYTDEARRSRLN RLYGIQGIPTLIMLDPQGEV ITRQGRVEVLNDEDCREFPW HPKPVLELSDSNAAQLNEGP CLVLFVDSEDDGESEAAKQL IQPIAEKIIAKYKAKEEEAP LLFFVAGEDDMTDSLRDYTN L
Function Functions as a redox-dependent negative regulator of the Wnt signaling pathway, possibly by preventing ubiquitination of DVL3 by the BCR(KLHL12) complex. May also function as a transcriptional regulator act as a regulator of protein phosphatase 2A (PP2A).
Protein Name Nucleoredoxin
Cellular Localisation Cytoplasm
Cytosol
Nucleus
Alternative Antibody Names Anti-Nucleoredoxin antibody
Anti-NXN antibody
Anti-NRX antibody

Information sourced from Uniprot.org

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