| Host: | Rabbit |
| Applications: | WB/ICC/IF |
| Reactivity: | Human/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit monoclonal anti-NUP50 for use in WB, ICC and IF in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | R02-3H1 |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Purification: | Affinity Purified |
| Concentration: | 0.3 mg/mL |
| Dilution Range: | WB 1:500-1:1000IF 1:50-1:200 |
| Storage Instruction: | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: | NUP50 |
| Gene ID: | 10762 |
| Uniprot ID: | NUP50_HUMAN |
| Immunogen: | A synthetic peptide of human NUP50 |
| Tissue Specificity | Ubiquitous. Highest levels in testis, peripheral blood leukocytes and fetal liver. |
| Function | Component of the nuclear pore complex that has a direct role in nuclear protein import. Actively displaces NLSs from importin-alpha, and facilitates disassembly of the importin-alpha:beta-cargo complex and importin recycling. Interacts with regulatory proteins of cell cycle progression including CDKN1B. This interaction is required for correct intracellular transport and degradation of CDKN1B. |
| Protein Name | Nuclear Pore Complex Protein Nup5050 Kda NucleoporinNuclear Pore-Associated Protein 60 Kda-LikeNucleoporin Nup50 |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-159227Reactome: R-HSA-159230Reactome: R-HSA-159231Reactome: R-HSA-159236Reactome: R-HSA-165054Reactome: R-HSA-168271Reactome: R-HSA-168276Reactome: R-HSA-168325Reactome: R-HSA-168333Reactome: R-HSA-170822Reactome: R-HSA-180746Reactome: R-HSA-180910Reactome: R-HSA-191859Reactome: R-HSA-3108214Reactome: R-HSA-3232142Reactome: R-HSA-3301854Reactome: R-HSA-3371453Reactome: R-HSA-4085377Reactome: R-HSA-4551638Reactome: R-HSA-4570464Reactome: R-HSA-4615885Reactome: R-HSA-5578749Reactome: R-HSA-5619107Reactome: R-HSA-6784531Reactome: R-HSA-9609690Reactome: R-HSA-9610379Reactome: R-HSA-9705671 |
| Cellular Localisation | NucleusNuclear Pore ComplexNucleus MembranePeripheral Membrane ProteinNucleoplasmic SideLocalizes To The Nucleoplasmic Fibrils Of The Nuclear Pore ComplexDissociates From The Npc Structure Early During Prophase Of MitosisAssociates With The Newly Formed Nuclear Membrane During TelophaseIn The TestisThe Localization Changes During Germ Cell Differentiation From The Nuclear Surface In Spermatocytes To The Whole Nucleus (Interior) In Spermatids And Back To The Nuclear Surface In Spermatozoa |
| Alternative Antibody Names | Anti-Nuclear Pore Complex Protein Nup50 antibodyAnti-50 Kda Nucleoporin antibodyAnti-Nuclear Pore-Associated Protein 60 Kda-Like antibodyAnti-Nucleoporin Nup50 antibodyAnti-NUP50 antibodyAnti-NPAP60L antibodyAnti-PRO1146 antibody |
Information sourced from Uniprot.org
