• Western blot analysis of various lysates using NUB1 Rabbit polyclonal antibody (STJ11101463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 300s.

Anti-NUB1 antibody (1-100) (STJ11101463)

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STJ11101463

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NUB1 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NUB1
Gene ID: 51667
Uniprot ID: NUB1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human IFT140 (NP_001350458.1).
Immunogen Sequence: MAQKKYLQAKLTQFLREDRI QLWKPPYTDENKKVGLALKD LAKQYSDRLECCENEVEKVI EEIRCKAIERGTGNDNYRTT GIATIEVFLPPRLKKDRKNL
Tissue Specificity Widely expressed with lowest expression in the pancreas for isoform 1 and in leukocytes, liver, prostate and skeletal muscle for isoform 2.
Function Specific down-regulator of the NEDD8 conjugation system. Recruits NEDD8, UBD, and their conjugates to the proteasome for degradation. Isoform 1 promotes the degradation of NEDD8 more efficiently than isoform 2.
Protein Name Nedd8 Ultimate Buster 1
Negative Regulator Of Ubiquitin-Like Proteins 1
Renal Carcinoma Antigen Ny-Ren-18
Database Links Reactome: R-HSA-8951664
Cellular Localisation Nucleus
Predominantly Nuclear
Alternative Antibody Names Anti-Nedd8 Ultimate Buster 1 antibody
Anti-Negative Regulator Of Ubiquitin-Like Proteins 1 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-18 antibody
Anti-NUB1 antibody
Anti-NYREN18 antibody

Information sourced from Uniprot.org

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