Immunoprecipitation analysis of 200 Mu g extracts of A-549 cells, using 3 Mu g NSFL1C antibody (STJ28760). Western blot was performed from the immunoprecipitate using NSFL1C antibody (STJ28760) at a dilution of 1:1000.

Anti-NSFL1C antibody (1-185) (STJ28760)

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STJ28760
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Short Description :Rabbit polyclonal NSFL1C (1-185) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
Applications:WB/IF/ICC/IP/ELISA
Host:Rabbit
Reactivity:Human/Mouse/Rat
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:500-1:2000
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements
Formulation:PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:NSFL1C
Gene ID:55968
Uniprot ID:NSF1C_HUMAN
Immunogen Region:1-185
Immunogen Sequence:MAAERQEALREFVAVTGAEE DRARFFLESAGWDLQIALAS FYEDGGDEDIVTISQATPSS VSRGTAPSDNRVTSFRDLIH DQDEDEEEEEGQRFYAGGSE RSGQQIVGPPRKKSPNELVD DLFKGAKEHGAVAVERVTKS PGETSKPRPFAGGGYRLGAA PEEESAYVAGEKRQHSSQDV HVVLK
Specificity:Recombinant fusion protein containing a sequence corresponding to amino acids 1-185 of human NSFL1C (NP_057227.2).
Post Translational Modifications Phosphorylated during mitosis. Phosphorylation inhibits interaction with Golgi membranes and is required for the fragmentation of the Golgi stacks during mitosis.
Function Reduces the ATPase activity of VCP. Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis. May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER). Inhibits the activity of CTSL (in vitro). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase. Also, regulates spindle orientation during mitosis.
Protein Name Nsfl1 Cofactor P47
Ubx Domain-Containing Protein 2c
P97 Cofactor P47
Database Links Reactome: R-HSA-9013407
Cellular Localisation Nucleus
Golgi Apparatus
Golgi Stack
Chromosome
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Predominantly Nuclear In Interphase Cells
Bound To The Axial Elements Of Sex Chromosomes In Pachytene Spermatocytes
A Small Proportion Of The Protein Is Cytoplasmic
Associated With Golgi Stacks
Localizes To Centrosome During Mitotic Prophase And Metaphase
Alternative Antibody Names Anti-Nsfl1 Cofactor P47 antibody
Anti-Ubx Domain-Containing Protein 2c antibody
Anti-P97 Cofactor P47 antibody
Anti-NSFL1C antibody
Anti-UBXN2C antibody

Information sourced from Uniprot.org

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