Anti-NSF antibody (251-400) (STJ29825)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NSF (251-400) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 400 Mu g-600 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirement |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NSF |
| Gene ID | 4905 |
| Uniprot ID | NSF_HUMAN |
| Immunogen Region | 251-400 |
| Immunogen Sequence | KHVKGILLYGPPGCGKTLLA RQIGKMLNAREPKVVNGPEI LNKYVGESEANIRKLFADAE EEQRRLGANSGLHIIIFDEI DAICKQRGSMAGSTGVHDTV VNQLLSKIDGVEQLNNILVI GMTNRPDLIDEALLRPGRLE VKMEIGLPDE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 251-400 of human NSF (NP_006169.2). |
Additional Info
| Post Translational Modifications | Phosphorylation at Ser-569 interferes with homohexamerization. |
| Function | Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling. |
| Protein Name | Vesicle-Fusing AtpaseN-Ethylmaleimide-Sensitive Fusion ProteinNem-Sensitive Fusion ProteinVesicular-Fusion Protein Nsf |
| Database Links | Reactome: R-HSA-204005Reactome: R-HSA-416993Reactome: R-HSA-6807878Reactome: R-HSA-6811434Reactome: R-HSA-6811438Reactome: R-HSA-6811440 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Vesicle-Fusing Atpase antibodyAnti-N-Ethylmaleimide-Sensitive Fusion Protein antibodyAnti-Nem-Sensitive Fusion Protein antibodyAnti-Vesicular-Fusion Protein Nsf antibodyAnti-NSF antibody |
Information sourced from Uniprot.org