| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NR2C1 (1-240) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NR2C1 |
| Gene ID: |
7181 |
| Uniprot ID: |
NR2C1_HUMAN |
| Immunogen Region: |
1-240 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human NR2C1 (NP_003288.2). |
| Immunogen Sequence: |
MATIEEIAHQIIEQQMGEIV TEQQTGQKIQIVTALDHNTQ GKQFILTNHDGSTPSKVILA RQDSTPGKVFLTTPDAAGVN QLFFTTPDLSAQHLQLLTDN SPDQGPNKVFDLCVVCGDKA SGRHYGAVTCEGCKGFFKRS IRKNLVYSCRGSKDCIINKH HRNRCQYCRLQRCIAFGMKQ DSVQCERKPIEVSREKSSNC AASTEKIYIRKDLRSPLTAT PTFVTDSESTRSTGLLDSG |
| Post Translational Modifications | Sumoylation requires both PIAS1 and UBE2I. Sumoylation appears to dissociate NR2C1 from the PML nuclear bodies. Enhances the interaction with NRIP1 but inhibits interaction with KAT2B. In proliferating cells, stimulation by all-trans retinoic acid, activation of MAPK1-mediated phosphorylation and recruitment to PML bodies with subsequent sumoylation, suppresses OCT4 expression. Phosphorylated on several serine and threonine residues. Phosphorylation on Thr-222, stimulated by all-trans retinoic acid (atRA) mediates PML location and sumoylation in proliferating cells which then modulates its association with effector molecules, KAT2B and NRIP1. Phosphorylation on Ser-581 by PKC is important for protein stability and function as activator of RARB. |
| Function | Orphan nuclear receptor. Binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. Primarily repressor of a broad range of genes. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Together with NR2C2, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription. Also activator of OCT4 gene expression. May be involved in stem cell proliferation and differentiation. Mediator of retinoic acid-regulated preadipocyte proliferation. |
| Protein Name | Nuclear Receptor Subfamily 2 Group C Member 1Orphan Nuclear Receptor Tr2Testicular Receptor 2 |
| Database Links | Reactome: R-HSA-383280Reactome: R-HSA-4090294 |
| Cellular Localisation | NucleusPml BodyRecruited By Hdac3After All-Trans Retinoic Acid Stimulated Mapk1-Mediated Thr-223 PhosphorylationTo Pml Bodies For Subsequent Sumoylation |
| Alternative Antibody Names | Anti-Nuclear Receptor Subfamily 2 Group C Member 1 antibodyAnti-Orphan Nuclear Receptor Tr2 antibodyAnti-Testicular Receptor 2 antibodyAnti-NR2C1 antibodyAnti-TR2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
