• Immunofluorescence analysis of NIH/3T3 cells using NR1H2 Rabbit polyclonal antibody (STJ11104520) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates, using NR1H2 Rabbit polyclonal antibody (STJ11104520) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NR1H2 antibody (180-260) (STJ11104520)

SKU:
STJ11104520

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Oxysterols Receptor Lxr-Beta (180-260) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NR1H2
Gene ID: 7376
Uniprot ID: NR1H2_HUMAN
Immunogen Region: 180-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 180-260 of human LXR beta/NER (NP_009052.4).
Immunogen Sequence: QSQSPVGPQGSSSSASGPGA SPGGSEAGSQGSGEGEGVQL TAAQELMIQQLVAAQLQCNK RSFSDQPKVTPWPLGADPQS R
Tissue Specificity Ubiquitous.
Post Translational Modifications Sumoylated by SUMO2 at Lys-409 and Lys-447 during the hepatic acute phase response, leading to promote interaction with GPS2 and prevent N-Cor corepressor complex dissociation.
Function Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.DLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism. Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis. Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex.
Protein Name Oxysterols Receptor Lxr-Beta
Liver X Receptor Beta
Nuclear Receptor Ner
Nuclear Receptor Subfamily 1 Group H Member 2
Ubiquitously-Expressed Nuclear Receptor
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-383280
Reactome: R-HSA-4090294
Reactome: R-HSA-8866427
Reactome: R-HSA-9029558
Reactome: R-HSA-9029569
Reactome: R-HSA-9031525
Reactome: R-HSA-9031528
Reactome: R-HSA-9623433
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Oxysterols Receptor Lxr-Beta antibody
Anti-Liver X Receptor Beta antibody
Anti-Nuclear Receptor Ner antibody
Anti-Nuclear Receptor Subfamily 1 Group H Member 2 antibody
Anti-Ubiquitously-Expressed Nuclear Receptor antibody
Anti-NR1H2 antibody
Anti-LXRB antibody
Anti-NER antibody
Anti-UNR antibody

Information sourced from Uniprot.org

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