Western blot analysis of various lysates using NPHP4 Rabbit pAb (STJ116333) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 30s.

Anti-NPHP4 antibody (1247-1426) (STJ116333)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ116333
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General Information

Short DescriptionRabbit polyclonal NPHP4 (1247-1426) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:3000
IF:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolNPHP4
Gene ID261734
Uniprot IDNPHP4_HUMAN
Immunogen Region1247-1426
Immunogen SequenceRLSLVLRGTQTVRKVRAFTS HPQELKTDPKGVFVLPPRGV QDLHVGVRPLRAGSRFVHLN LVDVDCHQLVASWLVCLCCR QPLISKAFEIMLAAGEGKGV NKRITYTNPYPSRRTFHLHS DHPELLRFREDSFQVGGGET YTIGLQFAPSQRVGEEEILI YINDHEDKNEEAFCVKVIYQ
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1247-1426 of human NPHP4 (NP_055917.1).

Additional Info

Tissue Specificity Expressed in kidney, skeletal muscle, heart and liver, and to a lesser extent in brain and lung.
Function Involved in the organization of apical junctions.the function is proposed to implicate a NPHP1-4-8 module. Does not seem to be strictly required for ciliogenesis. Required for building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells. Seems to recruit INT to basal bodies of motile cilia which subsequently interacts with actin-modifying proteins such as DAAM1. In cooperation with INVS may down-regulate the canonical Wnt pathway and promote the Wnt-PCP pathway by regulating expression and subcellular location of disheveled proteins. Stabilizes protein levels of JADE1 and promotes its translocation to the nucleus leading to cooperative inhibition of canonical Wnt signaling. Acts as a negative regulator of the hippo pathway by association with LATS1 and modifying LATS1-dependent phosphorylation and localization of WWTR1/TAZ.
Protein Name Nephrocystin-4
Nephroretinin
Database Links Reactome: R-HSA-2028269
Reactome: R-HSA-5620912
Cellular Localisation Cytoplasm
Cytoskeleton
Cilium Basal Body
Microtubule Organizing Center
Centrosome
Cell Junction
Tight Junction
Nucleus
In Cultured Renal Cells
It Localizes Diffusely In The Cytoplasm But
As Cells Approach Confluence
It Accumulates To Basolateral Tight Junctions
Localizes To The Ciliary Transition Zone
In The Retinal Photoreceptor Cell Layer
Localizes At The Connecting Cilium
Alternative Antibody Names Anti-Nephrocystin-4 antibody
Anti-Nephroretinin antibody
Anti-NPHP4 antibody
Anti-KIAA0673 antibody

Information sourced from Uniprot.org

Citations

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