| Host: | Rabbit |
| Applications: | WB |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Protein NPAT (629-679 aa) is suitable for use in Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000-2000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NPAT |
| Gene ID: | 4863 |
| Uniprot ID: | NPAT_HUMAN |
| Immunogen Region: | 629-679 aa |
| Specificity: | This antibody detects endogenous levels of human NPAT |
| Immunogen: | Synthesized peptide derived from the human NPAT at the amino acid range 629-679 |
| Post Translational Modifications | Phosphorylated at Ser-775, Ser-779, Ser-1100, Thr-1270 and Thr-1350 by CCNE1/CDK2 at G1-S transition and until prophase, which promotes association with histone gene clusters and stimulates activation of histone transcription. Also phosphorylated by CCNA1/CDK2 in vitro. |
| Function | Required for progression through the G1 and S phases of the cell cycle and for S phase entry. Activates transcription of the histone H2A, histone H2B, histone H3 and histone H4 genes in conjunction with MIZF. Also positively regulates the ATM, MIZF and PRKDC promoters. Transcriptional activation may be accomplished at least in part by the recruitment of the NuA4 histone acetyltransferase (HAT) complex to target gene promoters. |
| Protein Name | Protein Npat Nuclear Protein Of The Ataxia Telangiectasia Mutated Locus Nuclear Protein Of The Atm Locus P220 |
| Cellular Localisation | Nucleus Cajal Body Concentrates In Two Cajal Bodies Tethered To Histone Gene Clusters At Chromosome 6p21 During G1 S And G2 Phases Also Concentrates In Two Additional Cajal Bodies Tethered To Histone Gene Clusters At Chromosome 1q21 Specifically During S And G2 Phases |
| Alternative Antibody Names | Anti-Protein Npat antibody Anti-Nuclear Protein Of The Ataxia Telangiectasia Mutated Locus antibody Anti-Nuclear Protein Of The Atm Locus antibody Anti-P220 antibody Anti-NPAT antibody Anti-CAND3 antibody Anti-E14 antibody |
Information sourced from Uniprot.org