• Western blot analysis of 293T-NKX3-2, using NKX3-2 antibody (STJ111268) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of mouse spleen, using NKX3-2 antibody (STJ111268) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NKX3-2 antibody (1-180) (STJ111268)

SKU:
STJ111268

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NKX3-2 (1-180) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NKX3-2
Gene ID: 579
Uniprot ID: NKX32_HUMAN
Immunogen Region: 1-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human NKX3-2 (NP_001180.1).
Immunogen Sequence: MAVRGANTLTSFSIQAILNK KEERGGLAAPEGRPAPGGTA ASVAAAPAVCCWRLFGERDA GALGGAEDSLLASPAGTRTA AGRTAESPEGWDSDSALSEE NESRRRCADARGASGAGLAG GSLSLGQPVCELAASKDLEE EAAGRSDSEMSASVSGDRSP RTEDDGVGPRGAHVSALCSG
Tissue Specificity Expressed at highest levels in cartilage, bone (osteosarcoma) and gut (small intestine and colon), whereas moderate expression is seen in trachea and brain. Expressed in visceral mesoderm and embryonic skeleton.
Function Transcriptional repressor that acts as a negative regulator of chondrocyte maturation. PLays a role in distal stomach development.required for proper antral-pyloric morphogenesis and development of antral-type epithelium. In concert with GSC, defines the structural components of the middle ear.required for tympanic ring and gonium development and in the regulation of the width of the malleus.
Protein Name Homeobox Protein Nkx-3.2
Bagpipe Homeobox Protein Homolog 1
Homeobox Protein Nk-3 Homolog B
Database Links Reactome: R-HSA-8939902
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Nkx-3.2 antibody
Anti-Bagpipe Homeobox Protein Homolog 1 antibody
Anti-Homeobox Protein Nk-3 Homolog B antibody
Anti-NKX3-2 antibody
Anti-BAPX1 antibody
Anti-NKX3B antibody

Information sourced from Uniprot.org

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