Western blot analysis of extracts of LNCaP cells, using NKX3. 1 antibody (STJ11103370) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NKX3.1 antibody (1-80) (STJ11103370)

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STJ11103370
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NKX3-1
Gene ID: 4824
Uniprot ID: NKX31_HUMAN
Immunogen Region: 1-80
Specificity: Recombinant fusion protein containing a sequence within amino acids 1-80 of human NKX3.1 (NP_006158.2).
Immunogen Sequence: MLRVPEPRPGEAKAEGAAPP TPSKPLTSFLIQDILRDGAQ RQGGRTSSQRQRDPEPEPEP EPEGGRSRAGAQNDQLSTGP
Tissue Specificity Highly expressed in the prostate and, at a lower level, in the testis.
Post Translational Modifications Ubiquitinated by TOPORS.monoubiquitinated at several residues and also polyubiquitinated on single residues.
Function Transcription factor, which binds preferentially the consensus sequence 5'-TAAGTAG-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
Protein Name Homeobox Protein Nkx-3.1
Homeobox Protein Nk-3 Homolog A
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Homeobox Protein Nkx-3.1 antibody
Anti-Homeobox Protein Nk-3 Homolog A antibody
Anti-NKX3-1 antibody
Anti-NKX3.1 antibody
Anti-NKX3A antibody

Information sourced from Uniprot.org

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