• Western blot analysis of extracts of Jurkat cells, using NIN antibody (STJ110514) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. _Lysates/proteins: 25ug per lane. _Blocking buffer: 3% non-fat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using NIN antibody (STJ110514) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human stomach using NIN antibody (STJ110514) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using NIN antibody (STJ110514) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat kidney using NIN antibody (STJ110514) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using NIN antibody (STJ110514) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse lung using NIN antibody (STJ110514) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Jurkat cells, using NIN antibody (STJ110514) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-NIN antibody (1-287) (STJ110514)

SKU:
STJ110514

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NIN (1-287) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NIN
Gene ID: 51199
Uniprot ID: NIN_HUMAN
Immunogen Region: 1-287
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human NIN (NP_891991.1).
Immunogen Sequence: MDEVEQDQHEARLKELFDSF DTTGTGSLGQEELTDLCHML SLEEVAPVLQQTLLQDNLLG RVHFDQFKEALILILSRTLS NEEHFQEPDCSLEAQPKYVR GGKRYGRRSLPEFQESVEEF PEVTVIEPLDEEARPSHIPA GDCSEHWKTQRSEEYEAEGQ LRFWNPDDLNASQSGSSPPQ DWIEEKLQEVCEDLGITRDG HLNRKKLVSICEQYGLQNVD GEMLEEVFHNLDPDGTMSV
Tissue Specificity Ubiquitous. Highly expressed in heart and skeletal muscle. Isoform 1 is more expressed than isoform 5.
Post Translational Modifications Phosphorylated by AURKA/Aurora kinase A and PKA kinases but not CK2 or AURKB/ Aurora kinase B.
Function Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation, by recruiting the gamma-tubulin ring complex to the centrosome. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules. Required for centriole organization and microtubule anchoring at the mother centriole.
Protein Name Ninein
Hninein
Glycogen Synthase Kinase 3 Beta-Interacting Protein
Gsk3b-Interacting Protein
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Component Of The Core Centrosome
Arranged In A Tubular Conformation With An Open And A Closed End Within The Centrosome
In The Mother Centrosome
It Localizes At Both Ends Of The Centrosome Tube
Including The Site Of Centrosome Duplication
While In The Daughter Centrosome It Is Present Only At The Closed End
Requires Pcm1 For Centrosome Localization
Localizes To The Subdistal Appendage Region Of The Centriole In A Dctn1-Dependent Manner
Isoform 6: Cytoplasm
Seems To Have A Dominant-Negative Effect On Localization Of Other Isoforms
Promoting Their Dissociation From The Centrosome
Alternative Antibody Names Anti-Ninein antibody
Anti-Hninein antibody
Anti-Glycogen Synthase Kinase 3 Beta-Interacting Protein antibody
Anti-Gsk3b-Interacting Protein antibody
Anti-NIN antibody
Anti-KIAA1565 antibody

Information sourced from Uniprot.org

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