| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | Cspg4 |
| Gene ID: | 121021 |
| Uniprot ID: | CSPG4_MOUSE |
| Immunogen Region: | 1345-1572 aa |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1345-1572 of human NG2/CSPG4. (NP_620570.2). |
| Immunogen Sequence: | LGDPLEGISVELEVLPTVIP LDVQNFSVPEGGTRTLAPPL VQITGPYFPTLPGLVLQVLE PPQHGALQKEDHSQDGSLST FSWREVEEQLIRYVHDGSET QTDAFVLLANASEMDRQSQP VAFTITILPVNDQPPVLTTN TGLQIWEGAIVPIPPEALRG TDNDSGPEDLVYTIEQPSNG RIALRVAPDTEVHRFTQAQL DSGLVLFSHRGALEGGFHFD LSDGAHTS |
| Tissue Specificity | Expressed in microcascular pericytes and not endothelial cells. |
| Post Translational Modifications | O-glycosylated.contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation. Involved in interaction with MMP16 and ITGA4. Phosphorylation by PRKCA regulates its subcellular location and function in cell motility. |
| Function | Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. |
| Protein Name | Chondroitin Sulfate Proteoglycan 4 Chondroitin Sulfate Proteoglycan Ng2 Proteoglycan An2 |
| Database Links | Reactome: R-MMU-1971475 Reactome: -MMU-2022870 Reactome: -MMU-2022923 Reactome: -MMU-2024101 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Extracellular Side Apical Cell Membrane Cell Projection Lamellipodium Membrane Cell Surface Localized At The Apical Plasma Membrane It Relocalizes To The Lamellipodia Of Astrocytoma Upon Phosphorylation By Prkca Localizes To The Retraction Fibers A Fraction May Undergo Cell Surface Proteolysis And Secretion Localizes To The Plasma Membrane Of Oligodendrocytes |
| Alternative Antibody Names | Anti-Chondroitin Sulfate Proteoglycan 4 antibody Anti-Chondroitin Sulfate Proteoglycan Ng2 antibody Anti-Proteoglycan An2 antibody Anti-Cspg4 antibody Anti-An2 antibody Anti-Kiaa4232 antibody Anti-Ng2 antibody |
Information sourced from Uniprot.org
