| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Cspg4 |
| Gene ID: |
121021 |
| Uniprot ID: |
CSPG4_MOUSE |
| Immunogen Region: |
1345-1572 aa |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1345-1572 of human NG2/CSPG4. (NP_620570.2). |
| Immunogen Sequence: |
LGDPLEGISVELEVLPTVIP LDVQNFSVPEGGTRTLAPPL VQITGPYFPTLPGLVLQVLE PPQHGALQKEDHSQDGSLST FSWREVEEQLIRYVHDGSET QTDAFVLLANASEMDRQSQP VAFTITILPVNDQPPVLTTN TGLQIWEGAIVPIPPEALRG TDNDSGPEDLVYTIEQPSNG RIALRVAPDTEVHRFTQAQL DSGLVLFSHRGALEGGFHFD LSDGAHTS |
| Tissue Specificity | Expressed in microcascular pericytes and not endothelial cells. |
| Post Translational Modifications | O-glycosylated.contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation. Involved in interaction with MMP16 and ITGA4. Phosphorylation by PRKCA regulates its subcellular location and function in cell motility. |
| Function | Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. |
| Protein Name | Chondroitin Sulfate Proteoglycan 4Chondroitin Sulfate Proteoglycan Ng2Proteoglycan An2 |
| Database Links | Reactome: R-MMU-1971475Reactome: -MMU-2022870Reactome: -MMU-2022923Reactome: -MMU-2024101 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinExtracellular SideApical Cell MembraneCell ProjectionLamellipodium MembraneCell SurfaceLocalized At The Apical Plasma Membrane It Relocalizes To The Lamellipodia Of Astrocytoma Upon Phosphorylation By PrkcaLocalizes To The Retraction FibersA Fraction May Undergo Cell Surface Proteolysis And SecretionLocalizes To The Plasma Membrane Of Oligodendrocytes |
| Alternative Antibody Names | Anti-Chondroitin Sulfate Proteoglycan 4 antibodyAnti-Chondroitin Sulfate Proteoglycan Ng2 antibodyAnti-Proteoglycan An2 antibodyAnti-Cspg4 antibodyAnti-An2 antibodyAnti-Kiaa4232 antibodyAnti-Ng2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
