• Western blot analysis of 1) Jurkat Cell, 2) Mouse Brain Tissue lysate using IκB β mouse Monoclonal monoclonal antibody diluted at 1:2,000.
  • Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-NFKBIB antibody [3E4] (STJ196668)

SKU:
STJ196668

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-NF-kappa-B inhibitor beta is suitable for use in Immunohistochemistry and Western Blot research applications.
Clonality: Monoclonal
Clone ID: 3E4
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-P 1:50-200
WB 1:1000-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFKBIB
Gene ID: 4793
Uniprot ID: IKBB_HUMAN
Specificity: This antibody detects endogenous levels of I Kappa B Beta at Human, Mouse, Rat
Immunogen: Synthesized peptide derived from the human I Kappa B Beta
Post Translational Modifications Phosphorylated by RPS6KA1.followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
Function Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
Protein Name Nf-Kappa-B Inhibitor Beta
Nf-Kappa-Bib
I-Kappa-B-Beta
Ikb-B
Ikb-Beta
Ikappabbeta
Thyroid Receptor-Interacting Protein 9
Tr-Interacting Protein 9
Trip-9
Database Links Reactome: R-HSA-1169091
Reactome: R-HSA-1810476
Reactome: R-HSA-445989
Reactome: R-HSA-933542
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Nf-Kappa-B Inhibitor Beta antibody
Anti-Nf-Kappa-Bib antibody
Anti-I-Kappa-B-Beta antibody
Anti-Ikb-B antibody
Anti-Ikb-Beta antibody
Anti-Ikappabbeta antibody
Anti-Thyroid Receptor-Interacting Protein 9 antibody
Anti-Tr-Interacting Protein 9 antibody
Anti-Trip-9 antibody
Anti-NFKBIB antibody
Anti-IKBB antibody
Anti-TRIP9 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance