• Western blot analysis of extracts of various cell lines, using NFATC3 antibody (STJ28749) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using NFATC3 rabbit polyclonal antibody (STJ28749) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of Jurkat cells using NFATC3 rabbit polyclonal antibody (STJ28749) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of K-562 cells using NFATC3 rabbit polyclonal antibody (STJ28749) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-NFATC3 antibody (1-290) (STJ28749)

SKU:
STJ28749

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NFATC3 (1-290) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NFATC3
Gene ID: 4775
Uniprot ID: NFAC3_HUMAN
Immunogen Region: 1-290
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human NFATC3 (NP_775188.1).
Immunogen Sequence: MTTANCGAHDELDFKLVFGE DGAPAPPPPGSRPADLEPDD CASIYIFNVDPPPSTLTTPL CLPHHGLPSHSSVLSPSFQL QSHKNYEGTCEIPESKYSPL GGPKPFECPSIQITSISPNC HQELDAHEDDLQINDPEREF LERPSRDHLYLPLEPSYRES SLSPSPASSISSRSWFSDAS SCESLSHIYDDVDSELNEAA ARFTLGSPLTSPGGSPGGCP GEETWHQQYGLGHSLSPRQ
Tissue Specificity Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.
Post Translational Modifications Phosphorylated by NFATC-kinase.dephosphorylated by calcineurin.
Function Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2. Along with NFATC4, involved in embryonic heart development.
Protein Name Nuclear Factor Of Activated T-Cells - Cytoplasmic 3
Nf-Atc3
Nfatc3
Nfatx
T-Cell Transcription Factor Nfat4
Nf-At4
Nf-At4c
Database Links Reactome: R-HSA-2025928
Reactome: R-HSA-2871809
Reactome: R-HSA-5607763
Cellular Localisation Cytoplasm
Nucleus
Cytoplasmic For The Phosphorylated Form And Nuclear After Activation That Is Controlled By Calcineurin-Mediated Dephosphorylation
Rapid Nuclear Exit Of Nfatc Is Thought To Be One Mechanism By Which Cells Distinguish Between Sustained And Transient Calcium Signals
The Subcellular Localization Of Nfatc Plays A Key Role In The Regulation Of Gene Transcription
Alternative Antibody Names Anti-Nuclear Factor Of Activated T-Cells - Cytoplasmic 3 antibody
Anti-Nf-Atc3 antibody
Anti-Nfatc3 antibody
Anti-Nfatx antibody
Anti-T-Cell Transcription Factor Nfat4 antibody
Anti-Nf-At4 antibody
Anti-Nf-At4c antibody
Anti-NFATC3 antibody
Anti-NFAT4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance