| Host: |
Rabbit |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Nuclear factor of activated T-cells, cytoplasmic 2 (640-700 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000IHC-P 1:50-200ELISA 1:10000-20000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NFATC2 |
| Gene ID: |
4773 |
| Uniprot ID: |
NFAC2_HUMAN |
| Immunogen Region: |
640-700 aa |
| Specificity: |
The antibody detects endogenous NFATc2 |
| Immunogen: |
Synthetic peptide from the human protein at the amino acid range 640-700 |
| Post Translational Modifications | In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated.which is required for full transcriptional activity. Ubiquitinated in endothelial cells by RNF213 downstream of the non-canonical Wnt signaling pathway, leading to its degradation by the proteasome. |
| Function | Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. Is involved in the negative regulation of chondrogenesis. |
| Protein Name | Nuclear Factor Of Activated T-Cells - Cytoplasmic 2Nf-Atc2Nfatc2Nfat Pre-Existing SubunitNf-AtpT-Cell Transcription Factor Nfat1 |
| Database Links | Reactome: R-HSA-2025928Reactome: R-HSA-2871809Reactome: R-HSA-5607763Reactome: R-HSA-8877330 |
| Cellular Localisation | CytoplasmNucleusCytoplasmic For The Phosphorylated Form And Nuclear After Activation That Is Controlled By Calcineurin-Mediated DephosphorylationRapid Nuclear Exit Of Nfatc Is Thought To Be One Mechanism By Which Cells Distinguish Between Sustained And Transient Calcium SignalsThe Subcellular Localization Of Nfatc Plays A Key Role In The Regulation Of Gene Transcription |
| Alternative Antibody Names | Anti-Nuclear Factor Of Activated T-Cells - Cytoplasmic 2 antibodyAnti-Nf-Atc2 antibodyAnti-Nfatc2 antibodyAnti-Nfat Pre-Existing Subunit antibodyAnti-Nf-Atp antibodyAnti-T-Cell Transcription Factor Nfat1 antibodyAnti-NFATC2 antibodyAnti-NFAT1 antibodyAnti-NFATP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
