• Immunohistochemistry analysis of NEK9 in paraffin-embedded Human colon using NEK9 Rabbit polyclonal antibody (STJ119834) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of NEK9 in paraffin-embedded Rat ovary using NEK9 Rabbit polyclonal antibody (STJ119834) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using NEK9 Rabbit polyclonal antibody (STJ119834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-NEK9 antibody (730-979) (STJ119834)

SKU:
STJ119834

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NEK9 (730-979) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEK9
Gene ID: 91754
Uniprot ID: NEK9_HUMAN
Immunogen Region: 730-979
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 730-979 of human NEK9 (NP_149107.4).
Immunogen Sequence: SKTIRSNSSGLSIGTVFQSS SPGGGGGGGGGEEEDSQQES ETPDPSGGFRGTMEADRGME GLISPTEAMGNSNGASSSCP GWLRKELENAEFIPMPDSPS PLSAAFSESEKDTLPYEELQ GLKVASEAPLEHKPQVEASS PRLNPAVTCAGKGTPLTPPA CACSSLQVEVERLQGLVLKC LAEQQKLQQENLQIFTQLQK LNKKLEGGQQVGMHSKGTQT AKEEMEMDPKPDLDSDSWC
Tissue Specificity Most abundant in heart, liver, kidney and testis. Also expressed in smooth muscle cells and fibroblasts.
Post Translational Modifications Autophosphorylated on serine and threonine residues. When complexed with FACT, exhibits markedly elevated phosphorylation on Thr-210. During mitosis, not phosphorylated on Thr-210. Phosphorylated by CDK1 in vitro.
Function Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively.
Protein Name Serine/Threonine-Protein Kinase Nek9
Nercc1 Kinase
Never In Mitosis A-Related Kinase 9
Nima-Related Protein Kinase 9
Nima-Related Kinase 8
Nek8
Database Links Reactome: R-HSA-2980767
Reactome: R-HSA-3301854
Reactome: R-HSA-9648025
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase Nek9 antibody
Anti-Nercc1 Kinase antibody
Anti-Never In Mitosis A-Related Kinase 9 antibody
Anti-Nima-Related Protein Kinase 9 antibody
Anti-Nima-Related Kinase 8 antibody
Anti-Nek8 antibody
Anti-NEK9 antibody
Anti-KIAA1995 antibody
Anti-NEK8 antibody
Anti-NERCC antibody

Information sourced from Uniprot.org

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