• Western blot of rat cortical lysate showing specific immunolabeling of the ~68 kDa NF-L protein.
  • Immunofluorescence of a section of rat cerebellum showing specific labeling of Neurofilament-L (STJA0003700, 1:2000, green) and labeling of FOX3/NeuN ( cat. STJA0003841, 1:5000, red). The anti-NFL strongly labels the axons of basket calls and perikarya and processes of neuronal cells. The anti-FOX3/NeuN labels the nuclei and proximal cytoplasm of neurons. The blue is DAPI staining of nuclear DNA.
  • Immunostaining of mixed cultured rat neurons and glia stained with anti-NFL antibody (STJA0003700, red, 1:5000). The NF-L protein is assembled into neurofilaments which are found throughout the axons, dendrites, and perikarya as shown above.

Anti-NEFL antibody (STJA0003700)

SKU:
STJA0003700-100

$532.58
Shipping:
Free Shipping
Current Stock:
Host: Chicken
Applications: WB/IHC/ICC
Reactivity: Bovine/Chicken/Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Chicken polyclonal antibody anti-NEFL/NF-L is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgY
Formulation: Total IgY fraction in PBS + 10 mM Sodium Azide.
Purification: This antibody was total igy fraction.
Dilution Range: WB 1:10000
IHC 1:2000
ICC 1:2000-1:5000
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEFL
Gene ID: 4747
Uniprot ID: NFL_HUMAN
Immunogen: Preparation of bovine spinal cord NF-L.
Post Translational Modifications O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1.
Function Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Protein Name Neurofilament Light Polypeptide
Nf-L
68 Kda Neurofilament Protein
Neurofilament Triplet L Protein
Database Links Reactome: R-HSA-438066
Reactome: R-HSA-442982
Reactome: R-HSA-5673001
Reactome: R-HSA-9609736
Reactome: R-HSA-9617324
Reactome: R-HSA-9620244
Cellular Localisation Cell Projection
Axon
Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Neurofilament Light Polypeptide antibody
Anti-Nf-L antibody
Anti-68 Kda Neurofilament Protein antibody
Anti-Neurofilament Triplet L Protein antibody
Anti-NEFL antibody
Anti-NF68 antibody
Anti-NFL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance