• Western blot of rat cortical lysate showing specific immunolabeling of the ~200 kDa NF-H protein.
  • Immunostaining of human cerebellar cortex showing labeling of NF-H (STJA0003697, DAB, 1:500) in basket cell axons surrounding the large Purkinje neurons. Cerebellar granule cell layer is at the bottom of the image and the molecular layer at the top. Section was counterstained with Hematoxylin.

Anti-NEFH antibody [NAP4] (STJA0003697)

SKU:
STJA0003697-100

£394.50
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Host: Mouse
Applications: WB/IHC/ICC
Reactivity: All Mammalian/Chicken
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-NEFH/NF-H is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: NAP4
Conjugation: Unconjugated
Isotype: IgG1
Formulation: 100 ul in PBS + 10 mM Sodium Azide.
Purification: This antibody was protein g purified culture from supernatant.
Dilution Range: WB 1:5000
IHC 1:500-1:1000
ICC 1:500-1:1000
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEFH
Gene ID: 4744
Uniprot ID: NFH_HUMAN
Immunogen: purified bovine NF-H.
Post Translational Modifications There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Function Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Protein Name Neurofilament Heavy Polypeptide
Nf-H
200 Kda Neurofilament Protein
Neurofilament Triplet H Protein
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Axon
Alternative Antibody Names Anti-Neurofilament Heavy Polypeptide antibody
Anti-Nf-H antibody
Anti-200 Kda Neurofilament Protein antibody
Anti-Neurofilament Triplet H Protein antibody
Anti-NEFH antibody
Anti-KIAA0845 antibody
Anti-NFH antibody

Information sourced from Uniprot.org

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