Anti-NEFH antibody [NAP4] (STJA0003697)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
Immunogenpurified bovine NF-H.
General Information
| Short Description | Mouse monoclonal anti-NEFH/NF-H for use in WB, IHC and ICC in All Mammalian and Chicken samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/ICC |
| Host | Mouse |
| Reactivity | All Mammalian/Chicken |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | NAP4 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | This antibody was protein g purified culture from supernatant. |
| Dilution Range | WB 1:5000IHC 1:500-1:1000ICC 1:500-1:1000 |
| Formulation | 100 ul in PBS + 10 mM Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NEFH |
| Gene ID | 4744 |
| Uniprot ID | NFH_HUMAN |
| Immunogen | purified bovine NF-H. |
Additional Info
| Post Translational Modifications | There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. |
| Function | Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks. |
| Protein Name | Neurofilament Heavy PolypeptideNf-H200 Kda Neurofilament ProteinNeurofilament Triplet H Protein |
| Database Links | |
| Cellular Localisation | CytoplasmCytoskeletonCell ProjectionAxon |
| Alternative Antibody Names | Anti-Neurofilament Heavy Polypeptide antibodyAnti-Nf-H antibodyAnti-200 Kda Neurofilament Protein antibodyAnti-Neurofilament Triplet H Protein antibodyAnti-NEFH antibodyAnti-KIAA0845 antibodyAnti-NFH antibody |
Information sourced from Uniprot.org