• Western blot analysis of extracts of various cell lines, using Neurofilament H antibody (STJ110740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g Neurofilament H antibody (STJ110740). Western blot was performed from the immunoprecipitate using Neurofilament H antibody (STJ110740) at a dilution of 1:1000.
  • Immunofluorescence analysis of C6 cells using Neurofilament H rabbit polyclonal antibody (STJ110740) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded mouse brain using Neurofilament H Rabbit polyclonal antibody (STJ110740) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse brain using Neurofilament H rabbit polyclonal antibody (STJ110740) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Neurofilament H Rabbit polyclonal antibody (STJ110740) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 600ug extracts of mouse brain cells using 3ug Neurofilament H antibody (STJ110740). Western blot was performed from the immunoprecipitate using Neurofilament H antibody (STJ110740) at a dilition of 1:1000.
  • Western blot analysis of various lysates using Neurofilament H Rabbit polyclonal antibody (STJ110740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NEFH antibody (873-1020) (STJ110740)

SKU:
STJ110740

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NEFH (873-1020) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NEFH
Gene ID: 4744
Uniprot ID: NFH_HUMAN
Immunogen Region: 873-1020
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 873-1020 of human Neurofilament H (NP_066554.2).
Immunogen Sequence: KVEEKKEPAVEKPKESKVEA KKEEAEDKKKVPTPEKEAPA KVEVKEDAKPKEKTEVAKKE PDDAKAKEPSKPAEKKEAAP EKKDTKEEKAKKPEEKPKTE AKAKEDDKTLSKEPSKPKAE KAEKSSSTDQKDSKPPEKAT EDKAAKGK
Post Translational Modifications There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization.
Function Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Protein Name Neurofilament Heavy Polypeptide
Nf-H
200 Kda Neurofilament Protein
Neurofilament Triplet H Protein
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Axon
Alternative Antibody Names Anti-Neurofilament Heavy Polypeptide antibody
Anti-Nf-H antibody
Anti-200 Kda Neurofilament Protein antibody
Anti-Neurofilament Triplet H Protein antibody
Anti-NEFH antibody
Anti-KIAA0845 antibody
Anti-NFH antibody

Information sourced from Uniprot.org

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