• Western blot analysis of Mouse testis, using PVRL3 Rabbit polyclonal antibody (STJ11105695) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 45s.

Anti-NECTIN3 antibody (127-366) (STJ11105695)

SKU:
STJ11105695

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Nectin-3 (127-366) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NECTIN3
Gene ID: 25945
Uniprot ID: NECT3_HUMAN
Immunogen Region: 127-366
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 127-366 of human PVRL3 (NP_001230215.1).
Immunogen Sequence: SLNDATITLHNIGFSDSGKY ICKAVTFPLGNAQSSTTVTV LVEPTVSLIKGPDSLIDGGN ETVAAICIAATGKPVAHIDW EGDLGEMESTTTSFPNETAT IISQYKLFPTRFARGRRITC VVKHPALEKDIRYSFILDIQ YAPEVSVTGYDGNWFVGRKG VNLKCNADANPPPFKSVWSR LDGQWPDGLLASDNTLHFVH PLTFNYSGVYICKVTNSLGQ RSDQKVIYISAYNSVASLN
Tissue Specificity Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.
Function Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.
Protein Name Nectin-3
Cdw113
Nectin Cell Adhesion Molecule 3
Poliovirus Receptor-Related Protein 3
Cd Antigen Cd113
Database Links Reactome: R-HSA-418990
Reactome: R-HSA-420597
Cellular Localisation Cell Membrane
Single-Pass Membrane Protein
Postsynaptic Cell Membrane
Alternative Antibody Names Anti-Nectin-3 antibody
Anti-Cdw113 antibody
Anti-Nectin Cell Adhesion Molecule 3 antibody
Anti-Poliovirus Receptor-Related Protein 3 antibody
Anti-Cd Antigen Cd113 antibody
Anti-NECTIN3 antibody
Anti-PRR3 antibody
Anti-PVRL3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance