| Post Translational Modifications | Dimethylation at Arg-118 by NDUFAF7 takes place after NDUFS2 assembles into the complex I, leading to stabilize the early intermediate complex. |
| Function | Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity of complex I. Essential for the assembly of complex I. Redox-sensitive, critical component of the oxygen-sensing pathway in the pulmonary vasculature which plays a key role in acute pulmonary oxygen-sensing and hypoxic pulmonary vasoconstriction. Plays an important role in carotid body sensing of hypoxia. Essential for glia-like neural stem and progenitor cell proliferation, differentiation and subsequent oligodendrocyte or neuronal maturation. |
| Protein Name | Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 2 - MitochondrialComplex I-49kdCi-49kdNadh-Ubiquinone Oxidoreductase 49 Kda Subunit |
| Database Links | Reactome: R-HSA-611105Reactome: R-HSA-6799198 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinMatrix Side |
| Alternative Antibody Names | Anti-Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 2 - Mitochondrial antibodyAnti-Complex I-49kd antibodyAnti-Ci-49kd antibodyAnti-Nadh-Ubiquinone Oxidoreductase 49 Kda Subunit antibodyAnti-NDUFS2 antibody |
Information sourced from Uniprot.org
![Immunofluorescence analysis of L929 cells using [KO Validated] NDUFS2 Rabbit polyclonal antibody (STJ114724) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93811/375003/STJ114724_1__02265.1713141659.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] NDUFS2 Rabbit polyclonal antibody (STJ114724) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and NDUFS2 knockout (KO) 293T cells, using [KO Validated] NDUFS2 Rabbit polyclonal antibody (STJ114724) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93811/375004/STJ114724_2__47765.1713141660.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and NDUFS2 knockout (KO) 293T cells, using [KO Validated] NDUFS2 Rabbit polyclonal antibody (STJ114724) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.")
![Western blot analysis of various lysates using [KO Validated] NDUFS2 Rabbit polyclonal antibody (STJ114724) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/93811/375005/STJ114724_3__46239.1713141661.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] NDUFS2 Rabbit polyclonal antibody (STJ114724) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunofluorescence analysis of L929 cells using [KO Validated] NDUFS2 Rabbit polyclonal antibody (STJ114724) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/93811/375003/STJ114724_1__02265.1713141659.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] NDUFS2 Rabbit polyclonal antibody (STJ114724) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
