• Immunofluorescence analysis of L929 cells using [KO Validated] NDUFS2 Rabbit polyclonal antibody (STJ114724) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and NDUFS2 knockout (KO) 293T cells, using [KO Validated] NDUFS2 Rabbit polyclonal antibody (STJ114724) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
  • Western blot analysis of various lysates using [KO Validated] NDUFS2 Rabbit polyclonal antibody (STJ114724) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-NDUFS2 antibody (204-463) (STJ114724)

SKU:
STJ114724

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFS2 (204-463) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:3000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFS2
Gene ID: 4720
Uniprot ID: NDUS2_HUMAN
Immunogen Region: 204-463
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 204-463 of human NDUFS2 (NP_004541.1).
Immunogen Sequence: FEEREKMFEFYERVSGARMH AAYIRPGGVHQDLPLGLMDD IYQFSKNFSLRLDELEELLT NNRIWRNRTIDIGVVTAEEA LNYGFSGVMLRGSGIQWDLR KTQPYDVYDQVEFDVPVGSR GDCYDRYLCRVEEMRQSLRI IAQCLNKMPPGEIKVDDAKV SPPKRAEMKTSMESLIHHFK LYTEGYQVPPGATYTAIEAP KGEFGVYLVSDGSSRPYRCK IKAPGFAHLAGLDKMSKGH
Post Translational Modifications Dimethylation at Arg-118 by NDUFAF7 takes place after NDUFS2 assembles into the complex I, leading to stabilize the early intermediate complex.
Function Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for the catalytic activity of complex I. Essential for the assembly of complex I. Redox-sensitive, critical component of the oxygen-sensing pathway in the pulmonary vasculature which plays a key role in acute pulmonary oxygen-sensing and hypoxic pulmonary vasoconstriction. Plays an important role in carotid body sensing of hypoxia. Essential for glia-like neural stem and progenitor cell proliferation, differentiation and subsequent oligodendrocyte or neuronal maturation.
Protein Name Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 2 - Mitochondrial
Complex I-49kd
Ci-49kd
Nadh-Ubiquinone Oxidoreductase 49 Kda Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone Iron-Sulfur Protein 2 - Mitochondrial antibody
Anti-Complex I-49kd antibody
Anti-Ci-49kd antibody
Anti-Nadh-Ubiquinone Oxidoreductase 49 Kda Subunit antibody
Anti-NDUFS2 antibody

Information sourced from Uniprot.org

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