• Immunofluorescence analysis of L929 cells using NDUFB3 Rabbit polyclonal antibody (STJ116590) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NDUFB3 Rabbit polyclonal antibody (STJ116590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NDUFB3 antibody (1-98) (STJ116590)

SKU:
STJ116590

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFB3 (1-98) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFB3
Gene ID: 4709
Uniprot ID: NDUB3_HUMAN
Immunogen Region: 1-98
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-98 of human NDUFB3 (NP_002482.1).
Immunogen Sequence: MAHEHGHEHGHHKMELPDYR QWKIEGTPLETIQKKLAAKG LRDPWGRNEAWRYMGGFAKS VSFSDVFFKGFKWGFAAFVV AVGAEYYLESLNKDKKHH
Post Translational Modifications Methylation at His residues by METTL9 enhances complex I-mediated mitochondrial respiration.
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 3
Complex I-B12
Ci-B12
Nadh-Ubiquinone Oxidoreductase B12 Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Single-Pass Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Beta Subcomplex Subunit 3 antibody
Anti-Complex I-B12 antibody
Anti-Ci-B12 antibody
Anti-Nadh-Ubiquinone Oxidoreductase B12 Subunit antibody
Anti-NDUFB3 antibody

Information sourced from Uniprot.org

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