• Immunofluorescence analysis of L929 cells using NDUFAF4 Rabbit polyclonal antibody (STJ116557) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using NDUFAF4 Rabbit polyclonal antibody (STJ116557) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of NDUFAF4 in paraffin-embedded Human liver cancer using NDUFAF4 Rabbit polyclonal antibody (STJ116557) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of NDUFAF4 in paraffin-embedded Rat heart using NDUFAF4 Rabbit polyclonal antibody (STJ116557) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of NDUFAF4 in paraffin-embedded Mouse heart using NDUFAF4 Rabbit polyclonal antibody (STJ116557) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using NDUFAF4 Rabbit polyclonal antibody (STJ116557) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-NDUFAF4 antibody (1-170) (STJ116557)

SKU:
STJ116557

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFAF4 (1-170) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFAF4
Gene ID: 29078
Uniprot ID: NDUF4_HUMAN
Immunogen Region: 1-170
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human NDUFAF4 (NP_054884.1).
Immunogen Sequence: MGALVIRGIRNFNLENRAER EISKMKPSVAPRHPSTNSLL REQISLYPEVKGEIARKDEK LLSFLKDVYVDSKDPVSSLQ VKAAETCQEPKEFRLPKDHH FDMINIKSIPKGKISIVEAL TLLNNHKLFPETWTAEKIMQ EYQLEQKDVNSLLKYFVTFE VEIFPPEDKK
Post Translational Modifications Phosphorylated on serine. Prolactin stimulate serine phosphorylation.
Function Involved in the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). May be involved in cell proliferation and survival of hormone-dependent tumor cells. May be a regulator of breast tumor cell invasion.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Assembly Factor 4
Hormone-Regulated Proliferation-Associated Protein Of 20 Kda
Database Links Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion
Membrane
Lipid-Anchor
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Assembly Factor 4 antibody
Anti-Hormone-Regulated Proliferation-Associated Protein Of 20 Kda antibody
Anti-NDUFAF4 antibody
Anti-C6orf66 antibody
Anti-HRPAP20 antibody
Anti-HSPC125 antibody
Anti-My013 antibody

Information sourced from Uniprot.org

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