Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g NDUFA8 antibody (STJ114012). Western blot was performed from the immunoprecipitate using NDUFA8 antibody (STJ114012) at a dilution of 1:1000.

Anti-NDUFA8 antibody (33-172) (STJ114012)

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STJ114012
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Applications:WB/IF/ICC/IP/ELISA
Host:Rabbit
Reactivity:Human/Mouse/Rat
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality :Polyclonal
Isotype:IgG
Conjugation:Unconjugated
Concentration:Lot specific
Purification:Affinity purification
Dilution Range:WB:1:500-1:2000
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements
Formulation:PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage Instruction:Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol:NDUFA8
Gene ID:4702
Uniprot ID:NDUA8_HUMAN
Immunogen Region:33-172
Immunogen Sequence:GAQCDKPNKEFMLCRWEEKD PRRCLEEGKLVNKCALDFFR QIKRHCAEPFTEYWTCIDYT GQQLFRHCRKQQAKFDECVL DKLGWVRPDLGELSKVTKVK TDRPLPENPYHSRPRPDPSP EIEGDLQPATHGSRFYFWTK
Specificity:Recombinant fusion protein containing a sequence corresponding to amino acids 33-172 of human NDUFA8 (NP_055037.1).
Post Translational Modifications May contain intrachain disulfide bonds, as evidenced by its electrophoretic mobility under reducing vs non-reducing conditions.
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 8
Complex I-19kd
Ci-19kd
Complex I-Pgiv
Ci-Pgiv
Nadh-Ubiquinone Oxidoreductase 19 Kda Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Mitochondrion Intermembrane Space
Mitochondrion
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 8 antibody
Anti-Complex I-19kd antibody
Anti-Ci-19kd antibody
Anti-Complex I-Pgiv antibody
Anti-Ci-Pgiv antibody
Anti-Nadh-Ubiquinone Oxidoreductase 19 Kda Subunit antibody
Anti-NDUFA8 antibody

Information sourced from Uniprot.org

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