• Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g NDUFA8 antibody (STJ114012). Western blot was performed from the immunoprecipitate using NDUFA8 antibody (STJ114012) at a dilution of 1:1000.
  • Immunofluorescence analysis of L929 cells using NDUFA8 Rabbit polyclonal antibody (STJ114012) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of H9C2 cells using NDUFA8 Rabbit polyclonal antibody (STJ114012) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NDUFA8 Rabbit polyclonal antibody (STJ114012) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NDUFA8 antibody (33-172) (STJ114012)

SKU:
STJ114012

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFA8
Gene ID: 4702
Uniprot ID: NDUA8_HUMAN
Immunogen Region: 33-172
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 33-172 of human NDUFA8 (NP_055037.1).
Immunogen Sequence: GAQCDKPNKEFMLCRWEEKD PRRCLEEGKLVNKCALDFFR QIKRHCAEPFTEYWTCIDYT GQQLFRHCRKQQAKFDECVL DKLGWVRPDLGELSKVTKVK TDRPLPENPYHSRPRPDPSP EIEGDLQPATHGSRFYFWTK
Post Translational Modifications May contain intrachain disulfide bonds, as evidenced by its electrophoretic mobility under reducing vs non-reducing conditions.
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 8
Complex I-19kd
Ci-19kd
Complex I-Pgiv
Ci-Pgiv
Nadh-Ubiquinone Oxidoreductase 19 Kda Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Mitochondrion Intermembrane Space
Mitochondrion
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 8 antibody
Anti-Complex I-19kd antibody
Anti-Ci-19kd antibody
Anti-Complex I-Pgiv antibody
Anti-Ci-Pgiv antibody
Anti-Nadh-Ubiquinone Oxidoreductase 19 Kda Subunit antibody
Anti-NDUFA8 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance