Anti-NDUFA8 antibody (33-172) (STJ114012)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NDUFA8 (33-172) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NDUFA8 |
| Gene ID | 4702 |
| Uniprot ID | NDUA8_HUMAN |
| Immunogen Region | 33-172 |
| Immunogen Sequence | GAQCDKPNKEFMLCRWEEKD PRRCLEEGKLVNKCALDFFR QIKRHCAEPFTEYWTCIDYT GQQLFRHCRKQQAKFDECVL DKLGWVRPDLGELSKVTKVK TDRPLPENPYHSRPRPDPSP EIEGDLQPATHGSRFYFWTK |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 33-172 of human NDUFA8 (NP_055037.1). |
Additional Info
| Post Translational Modifications | May contain intrachain disulfide bonds, as evidenced by its electrophoretic mobility under reducing vs non-reducing conditions. |
| Function | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. |
| Protein Name | Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 8Complex I-19kdCi-19kdComplex I-PgivCi-PgivNadh-Ubiquinone Oxidoreductase 19 Kda Subunit |
| Database Links | Reactome: R-HSA-611105Reactome: R-HSA-6799198 |
| Cellular Localisation | Mitochondrion Inner MembranePeripheral Membrane ProteinMitochondrion Intermembrane SpaceMitochondrion |
| Alternative Antibody Names | Anti-Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 8 antibodyAnti-Complex I-19kd antibodyAnti-Ci-19kd antibodyAnti-Complex I-Pgiv antibodyAnti-Ci-Pgiv antibodyAnti-Nadh-Ubiquinone Oxidoreductase 19 Kda Subunit antibodyAnti-NDUFA8 antibody |
Information sourced from Uniprot.org