Western blot analysis of extracts of various cell lines, using NDUFA5 antibody (STJ24717) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NDUFA5 antibody (1-116) (STJ24717)

SKU:
STJ24717
£112.50 - £524.50
Free Shipping
Processing The item has been added
Enquire For Bulk Order

Covered by St John's Laboratory Guarantee

Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFA5
Gene ID: 4698
Uniprot ID: NDUA5_HUMAN
Immunogen Region: 1-116
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human NDUFA5 (NP_004991.1).
Immunogen Sequence: MAGVLKKTTGLVGLAVCNTP HERLRILYTKILDVLEEIPK NAAYRKYTEQITNEKLAMVK AEPDVKKLEDQLQGGQLEEV ILQAEHELNLARKMREWKLW EPLVEEPPADQWKWPI
Tissue Specificity Expressed in all tissues examined with highest levels in heart, skeletal muscle and brain.
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 5
Complex I Subunit B13
Complex I-13kd-B
Ci-13kd-B
Nadh-Ubiquinone Oxidoreductase 13 Kda-B Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Reactome: R-HSA-9013408
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 5 antibody
Anti-Complex I Subunit B13 antibody
Anti-Complex I-13kd-B antibody
Anti-Ci-13kd-B antibody
Anti-Nadh-Ubiquinone Oxidoreductase 13 Kda-B Subunit antibody
Anti-NDUFA5 antibody

Information sourced from Uniprot.org

Related Products

Model Name Host Clonality Reactivity Application
STJ116951 Anti-NDUFA5 antibody (1-116) (STJ116951) Rabbit Polyclonal Human/Mouse/Rat WB/IHC-P/IF/ICC/ELISA
STJ11100321 Anti-SMCP antibody (1-116) (STJ11100321) Rabbit Polyclonal Human WB/ELISA
STJ117160 Anti-DNAJC19 antibody (1-116) (STJ117160) Rabbit Polyclonal Human/Mouse/Rat WB/IF/ICC/ELISA
STJ118713 Anti-DPPA5 antibody (1-116) (STJ118713) Rabbit Polyclonal Mouse/Rat WB/ELISA
STJ112130 Anti-IL1RL2 antibody (1-116) (STJ112130) Rabbit Polyclonal Human/Mouse WB/IF/ICC/ELISA
STJ26213 Anti-PPP1R1B antibody (1-116) (STJ26213) Rabbit Polyclonal Human/Mouse/Rat WB/IF/ICC/ELISA
STJ116583 Anti-VAMP5 antibody (1-116) (STJ116583) Rabbit Polyclonal Mouse WB/ELISA
STJ27128 Anti-DNAJC19 antibody (1-116) (STJ27128) Rabbit Polyclonal Human/Mouse/Rat WB/IF/ICC/ELISA
STJ191025 Anti-NDUFA5 antibody (10-90 aa) (STJ191025) Rabbit Polyclonal Human/Mouse WB/ELISA
!