• Immunofluorescence analysis of NIH-3T3 cells using NDUFA5 Polyclonal Antibody (STJ116951) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human gastric cancer using NDUFA5 antibody (STJ116951) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using NDUFA5 antibody (STJ116951) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human tonsil using NDUFA5 antibody (STJ116951) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NDUFA5 antibody (STJ116951) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NDUFA5 antibody (1-116) (STJ116951)

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STJ116951

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDUFA5 (1-116) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDUFA5
Gene ID: 4698
Uniprot ID: NDUA5_HUMAN
Immunogen Region: 1-116
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-116 of human NDUFA5 (NP_004991.1).
Immunogen Sequence: MAGVLKKTTGLVGLAVCNTP HERLRILYTKILDVLEEIPK NAAYRKYTEQITNEKLAMVK AEPDVKKLEDQLQGGQLEEV ILQAEHELNLARKMREWKLW EPLVEEPPADQWKWPI
Tissue Specificity Expressed in all tissues examined with highest levels in heart, skeletal muscle and brain.
Function Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Protein Name Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 5
Complex I Subunit B13
Complex I-13kd-B
Ci-13kd-B
Nadh-Ubiquinone Oxidoreductase 13 Kda-B Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-6799198
Reactome: R-HSA-9013408
Cellular Localisation Mitochondrion Inner Membrane
Peripheral Membrane Protein
Matrix Side
Alternative Antibody Names Anti-Nadh Dehydrogenase Ubiquinone 1 Alpha Subcomplex Subunit 5 antibody
Anti-Complex I Subunit B13 antibody
Anti-Complex I-13kd-B antibody
Anti-Ci-13kd-B antibody
Anti-Nadh-Ubiquinone Oxidoreductase 13 Kda-B Subunit antibody
Anti-NDUFA5 antibody

Information sourced from Uniprot.org

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