• Immunofluorescence analysis of L929 cells using NDRG4 Rabbit polyclonal antibody (STJ118635) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using NDRG4 Rabbit polyclonal antibody (STJ118635) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using NDRG4 Rabbit polyclonal antibody (STJ118635) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NDRG4 Rabbit polyclonal antibody (STJ118635) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NDRG4 antibody (140-220) (STJ118635)

SKU:
STJ118635

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDRG4 (140-220) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDRG4
Gene ID: 65009
Uniprot ID: NDRG4_HUMAN
Immunogen Region: 140-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 140-220 of human NDRG4 (NP_001229765.1).
Immunogen Sequence: DPNGKGWIDWAATKLSGLTS TLPDTVLSHLFSQEELVNNT ELVQSYRQQIGNVVNQANLQ LFWNMYNSRRDLDINRPGTV P
Tissue Specificity Expressed predominantly in brain and heart (at protein level). In the brain, detected in astrocytes. Isoform 1 and isoform 2 are only expressed in brain. Isoform 3 is expressed in both heart and brain. Up-regulated in glioblastoma multiforme cells.
Post Translational Modifications Phosphorylated in an aortic smooth muscle cell line, following PDGF treatment.
Function Contributes to the maintenance of intracerebral BDNF levels within the normal range, which is necessary for the preservation of spatial learning and the resistance to neuronal cell death caused by ischemic stress. May enhance growth factor-induced ERK1 and ERK2 phosphorylation, including that induced by PDGF and FGF. May attenuate NGF-promoted ELK1 phosphorylation in a microtubule-dependent manner.
Protein Name Protein Ndrg4
Brain Development-Related Molecule 1
N-Myc Downstream-Regulated Gene 4 Protein
Vascular Smooth Muscle Cell-Associated Protein 8
Smap-8
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Protein Ndrg4 antibody
Anti-Brain Development-Related Molecule 1 antibody
Anti-N-Myc Downstream-Regulated Gene 4 Protein antibody
Anti-Vascular Smooth Muscle Cell-Associated Protein 8 antibody
Anti-Smap-8 antibody
Anti-NDRG4 antibody
Anti-BDM1 antibody
Anti-KIAA1180 antibody

Information sourced from Uniprot.org

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