• Western blot analysis of extracts of various cell lines, using NUDEL/NUDEL/NDEL1 antibody (STJ28343) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NDEL1 antibody (1-345) (STJ28343)

SKU:
STJ28343

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDEL1 (1-345) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDEL1
Gene ID: 81565
Uniprot ID: NDEL1_HUMAN
Immunogen Region: 1-345
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-345 of human NUDEL/NUDEL/NDEL1 (NP_110435.1).
Immunogen Sequence: MDGEDIPDFSSLKEETAYWK ELSLKYKQSFQEARDELVEF QEGSRELEAELEAQLVQAEQ RNRDLQADNQRLKYEVEALK EKLEHQYAQSYKQVSVLEDD LSQTRAIKEQLHKYVRELEQ ANDDLERAKRATIVSLEDFE QRLNQAIERNAFLESELDEK ESLLVSVQRLKDEARDLRQE LAVRERQQEVTRKSAPSSPT LDCEKMDSAVQASLSLPATP VGKGTENTFPSPKAIPNGF
Tissue Specificity Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
Post Translational Modifications Phosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE. Palmitoylation at Cys-273 reduces affinity for dynein.
Function Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts.
Protein Name Nuclear Distribution Protein Nude-Like 1
Protein Nudel
Mitosin-Associated Protein 1
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Chromosome
Centromere
Kinetochore
Spindle
Localizes To The Cell Body Of The Motor Neurons And Colocalizes With Assembled Neurofilaments Within Axonal Processes
Localizes To The Microtubules Of The Manchette In Elongated Spermatids
Colocalizes With Disc1 In The Perinuclear Region
Including The Centrosome
Localizes To The Interphase Centrosome And The Mitotic Spindle
Localizes To The Kinetochore In A Cenpf-Dependent Manner
Alternative Antibody Names Anti-Nuclear Distribution Protein Nude-Like 1 antibody
Anti-Protein Nudel antibody
Anti-Mitosin-Associated Protein 1 antibody
Anti-NDEL1 antibody
Anti-EOPA antibody
Anti-MITAP1 antibody
Anti-NUDEL antibody

Information sourced from Uniprot.org

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