| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S6MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | NDC80 |
| Gene ID: | 10403 |
| Uniprot ID: | NDC80_HUMAN |
| Immunogen Region: | 543-642 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 543-642 of human HEC1/NDC80 (O14777). |
| Immunogen Sequence: | ESTVNQGLSEAMNELDAVQR EYQLVVQTTTEERRKVGNNL QRLLEMVATHVGSVEKHLEE QIAKVDREYEECMSEDLSEN IKEIRDKYEKKATLIKSSEE |
| Post Translational Modifications | Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. Also phosphorylated by AURKA and AURKB. Acetylated at Lys-53 and Lys-59 by KAT5 during mitosis, promoting robust kinetochore-microtubule attachment. Deacetylated by SIRT1. |
| Function | Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules. |
| Protein Name | Kinetochore Protein Ndc80 Homolog Highly Expressed In Cancer Protein Kinetochore Protein Hec1 Hshec1 Kinetochore-Associated Protein 2 Retinoblastoma-Associated Protein Hec |
| Database Links | Reactome: R-HSA-141444 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-5663220 Reactome: R-HSA-68877 Reactome: R-HSA-9648025 |
| Cellular Localisation | Nucleus Chromosome Centromere Kinetochore Localizes To Kinetochores From Late Prophase To Anaphase Localizes Specifically To The Outer Plate Of The Kinetochore |
| Alternative Antibody Names | Anti-Kinetochore Protein Ndc80 Homolog antibody Anti-Highly Expressed In Cancer Protein antibody Anti-Kinetochore Protein Hec1 antibody Anti-Hshec1 antibody Anti-Kinetochore-Associated Protein 2 antibody Anti-Retinoblastoma-Associated Protein Hec antibody Anti-NDC80 antibody Anti-HEC antibody Anti-HEC1 antibody Anti-KNTC2 antibody |
Information sourced from Uniprot.org