Western blot analysis of various lysates, using NCS1 antibody (STJ11102695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NCS1 antibody (91-190) [S5MR] (STJ11102695)

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STJ11102695
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Monoclonal
Clone ID : S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCS1
Gene ID: 23413
Uniprot ID: NCS1_HUMAN
Immunogen Region: 91-190
Specificity: A synthetic peptide corresponding to a sequence within amino acids 91-190 of human NCS1 (P62166).
Immunogen Sequence: VTSRGTLDEKLRWAFKLYDL DNDGYITRNEMLDIVDAIYQ MVGNTVELPEEENTPEKRVD RIFAMMDKNADGKLTLQEFQ EGSKADPSIVQALSLYDGLV
Function Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin. Stimulates PI4KB kinase activity. Involved in long-term synaptic plasticity through its interaction with PICK1. May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.
Protein Name Neuronal Calcium Sensor 1
Ncs-1
Frequenin Homolog
Frequenin-Like Protein
Frequenin-Like Ubiquitous Protein
Cellular Localisation Golgi Apparatus
Postsynaptic Density
Cytoplasm
Perinuclear Region
Cell Membrane
Peripheral Membrane Protein
Membrane
Lipid-Anchor
Associated With Golgi Stacks
Post-Synaptic Densities Of Dendrites
And In The Pre-Synaptic Nerve Terminal At Neuromuscular Junctions
Alternative Antibody Names Anti-Neuronal Calcium Sensor 1 antibody
Anti-Ncs-1 antibody
Anti-Frequenin Homolog antibody
Anti-Frequenin-Like Protein antibody
Anti-Frequenin-Like Ubiquitous Protein antibody
Anti-NCS1 antibody
Anti-FLUP antibody
Anti-FREQ antibody

Information sourced from Uniprot.org

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