• Immunofluorescence analysis of A549 cells using NCS1 antibody (STJ115547). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using NCS1 antibody (STJ115547) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NCS1 antibody (1-190) (STJ115547)

SKU:
STJ115547

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NCS1 (1-190) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCS1
Gene ID: 23413
Uniprot ID: NCS1_HUMAN
Immunogen Region: 1-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human NCS1 (NP_055101.2).
Immunogen Sequence: MGKSNSKLKPEVVEELTRKT YFTEKEVQQWYKGFIKDCPS GQLDAAGFQKIYKQFFPFGD PTKFATFVFNVFDENKDGRI EFSEFIQALSVTSRGTLDEK LRWAFKLYDLDNDGYITRNE MLDIVDAIYQMVGNTVELPE EENTPEKRVDRIFAMMDKNA DGKLTLQEFQEGSKADPSIV QALSLYDGLV
Function Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin. Stimulates PI4KB kinase activity. Involved in long-term synaptic plasticity through its interaction with PICK1. May also play a role in neuron differentiation through inhibition of the activity of N-type voltage-gated calcium channel.
Protein Name Neuronal Calcium Sensor 1
Ncs-1
Frequenin Homolog
Frequenin-Like Protein
Frequenin-Like Ubiquitous Protein
Cellular Localisation Golgi Apparatus
Postsynaptic Density
Cytoplasm
Perinuclear Region
Cell Membrane
Peripheral Membrane Protein
Membrane
Lipid-Anchor
Associated With Golgi Stacks
Post-Synaptic Densities Of Dendrites
And In The Pre-Synaptic Nerve Terminal At Neuromuscular Junctions
Alternative Antibody Names Anti-Neuronal Calcium Sensor 1 antibody
Anti-Ncs-1 antibody
Anti-Frequenin Homolog antibody
Anti-Frequenin-Like Protein antibody
Anti-Frequenin-Like Ubiquitous Protein antibody
Anti-NCS1 antibody
Anti-FLUP antibody
Anti-FREQ antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance