Anti-NCL antibody (500aa C-Term) (STJ140144)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
ImmunogenPurified recombinant peptide derived from within residues 500 aa to the C-terminus of human NCL produced in E. coli.
General Information
| Short Description | Goat polyclonal Nucleolin (500aa C-Term) antibody for use in WB in human, mouse, rat, bovine, canine, chicken, avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep and simian samples. Datasheet included with dilution recomm |
| Applications | WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Bovine/Canine/Chicken/Avian/Donkey/Feline/Goat/Guinea Pig/Hamster/Horse/Porcine/Rabbit/Sheep/Simian |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | This antibody is epitope-affinity purified from goat antiserum. |
| Dilution Range | WB 1:1000-1:5000 |
| Formulation | PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Storage Instruction | For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
Target Information
| Gene Symbol | NCL |
| Gene ID | 4691 |
| Uniprot ID | NUCL_HUMAN |
| Accession Number | ENSG00000115053 |
| Immunogen | Purified recombinant peptide derived from within residues 500 aa to the C-terminus of human NCL produced in E. coli. |
| Immunogen Region | 500aa C-Term |
| Immunogen Sequence | TLQEVFEKATFIKVPQNQNG LQEVFEKATFIKVPQNQNGK QEVFEKATFIKVPQNQNGKS EVFEKATFIKVPQNQNGKSK VFEKATFIKVPQNQNGKSKG FEKATFIKVPQNQNGKSKGY EKATFIKVPQNQNGKSKGYA KATFIKVPQNQNGKSKGYAF ATFIKVPQNQNGKSKGYAFI TFIKVPQNQNGKSKGYAFIE FIKVPQNQNGKSKGYAFIEF |
| Specificity | Detects endogenous levels of NCL by Western blot. |
Additional Info
| Post Translational Modifications | Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group. Symmetrically methylated by PRMT5. |
| Function | Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats. |
| Protein Name | NucleolinProtein C23 |
| Database Links | Reactome: R-HSA-6791226Reactome: R-HSA-9820960 |
| Cellular Localisation | NucleusNucleolusCytoplasmLocalized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas |
| Alternative Antibody Names | Anti-Nucleolin antibodyAnti-Protein C23 antibodyAnti-NCL antibody |
Information sourced from Uniprot.org