• Immunoprecipitation analysis of 200 Mu g extracts of Raji cells, using 3 Mu g p47phox/NCF1 antibody (STJ24698). Western blot was performed from the immunoprecipitate using p47phox/NCF1 antibody (STJ24698) at a dilution of 1:1000.
  • Western blot analysis of lysates from Raji cells using p47phox/NCF1 Rabbit polyclonal antibody (STJ24698) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:10s.
  • Western blot analysis of various lysates, using p47phox/NCF1 Rabbit polyclonal antibody (STJ24698) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-NCF1 antibody (1-390) (STJ24698)

SKU:
STJ24698

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NCF1 (1-390) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:100
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCF1
Gene ID: 653361
Uniprot ID: NCF1_HUMAN
Immunogen Region: 1-390
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-390 of human p47phox/NCF1 (NP_000256.4).
Immunogen Sequence: MGDTFIRHIALLGFEKRFVP SQHYVYMFLVKWQDLSEKVV YRRFTEIYEFHKTLKEMFPI EAGAINPENRIIPHLPAPKW FDGQRAAENRQGTLTEYCST LMSLPTKISRCPHLLDFFKV RPDDLKLPTDNQTKKPETYL MPKDGKSTATDITGPIILQT YRAIANYEKTSGSEMALSTG DVVEVVEKSESGWWFCQMKA KRGWIPASFLEPLDSPDETE DPEPNYAGEPYVAIKAYTA
Tissue Specificity Detected in peripheral blood monocytes and neutrophils (at protein level).
Post Translational Modifications Phosphorylated by PRKCD.phosphorylation induces activation of NCF1 and NADPH oxidase activity.
Function NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Protein Name Neutrophil Cytosol Factor 1
Ncf-1
47 Kda Autosomal Chronic Granulomatous Disease Protein
47 Kda Neutrophil Oxidase Factor
Ncf-47k
Neutrophil Nadph Oxidase Factor 1
Nox Organizer 2
Nox-Organizing Protein 2
Sh3 And Px Domain-Containing Protein 1a
P47-Phox
Database Links Reactome: R-HSA-1222556
Reactome: R-HSA-1236973
Reactome: R-HSA-3299685
Reactome: R-HSA-4420097
Reactome: R-HSA-5668599
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013423
Cellular Localisation Cytoplasm
Cytosol
Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Neutrophil Cytosol Factor 1 antibody
Anti-Ncf-1 antibody
Anti-47 Kda Autosomal Chronic Granulomatous Disease Protein antibody
Anti-47 Kda Neutrophil Oxidase Factor antibody
Anti-Ncf-47k antibody
Anti-Neutrophil Nadph Oxidase Factor 1 antibody
Anti-Nox Organizer 2 antibody
Anti-Nox-Organizing Protein 2 antibody
Anti-Sh3 And Px Domain-Containing Protein 1a antibody
Anti-P47-Phox antibody
Anti-NCF1 antibody
Anti-NOXO2 antibody
Anti-SH3PXD1A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance