• Immunohistochemistry analysis of paraffin-embedded mouse brain using NCAM1/ CD56 Rabbit polyclonal antibody (STJ24694) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using NCAM1/ CD56 Rabbit polyclonal antibody (STJ24694) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of mouse brain, using NCAM1/ CD56 antibody (STJ24694). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-NCAM1 antibody (759-858) (STJ24694)

SKU:
STJ24694

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NCAM1
Gene ID: 4684
Uniprot ID: NCAM1_HUMAN
Immunogen Region: 759-858
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 759-858 of human NCAM1/CD56 (NP_851996.2).
Immunogen Sequence: LCGKAGPGAKGKDMEEGKAA FSKDESKEPIVEVRTEEERT PNHDGGKHTEPNETTPLTEP EKGPVEAKPECQETETKPAP AEVKTVPNDATQTKENESKA
Post Translational Modifications Polysialylated at Asn-459 and Asn-488 by ST8SIA2 and ST8SIA4. Polysialylation modulates cell interactions by confering both attractive and repulsive properties that are highly regulated by ST8SIA2 and ST8SIA4. Polysialylation is formed on a-2,3-linked sialic acid of core glycans.
Function This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. (Microbial infection) Acts as a receptor for rabies virus. (Microbial infection) Acts as a receptor for Zika virus.
Protein Name Neural Cell Adhesion Molecule 1
N-Cam-1
Ncam-1
Cd Antigen Cd56
Database Links Reactome: R-HSA-3000178
Reactome: R-HSA-375165
Reactome: R-HSA-419037
Reactome: R-HSA-445144
Reactome: R-HSA-5673001
Reactome: R-HSA-877300
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Cell Membrane
Isoform 3: Cell Membrane
Lipid-Anchor
Gpi-Anchor
Isoform 4: Cell Membrane
Isoform 5: Secreted
Isoform 6: Secreted
Alternative Antibody Names Anti-Neural Cell Adhesion Molecule 1 antibody
Anti-N-Cam-1 antibody
Anti-Ncam-1 antibody
Anti-Cd Antigen Cd56 antibody
Anti-NCAM1 antibody
Anti-NCAM antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance